Myc tag Monoclonal Antibody

中文名称：

Myc tag鼠单克隆抗体
货号：

CSB-MA000041M0m
规格：

￥400
图片：
WB: Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6μg/ml
Lane 1: Recombinant Myc-tagged fusion protein at 50ng
Lane 2: Recombinant Myc-tagged fusion protein at 25ng
Lane 3: Recombinant Myc-tagged fusion protein at 12.25ng
Lane 4: Recombinant Myc-tagged fusion protein at 6.25ng
Lane 5: Recombinant Myc-tagged fusion protein at 3.125ng
Lane 6: Recombinant Myc-tagged fusion protein at 1.5625ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 50 kd
Observed band size: 50 kd

Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunoprecipitating MYC-tag with transfected HEK293
Lane 1: Mouse control IgG (1µg) instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA000041M0m (6µg) + transfected HEK293 whole cell lysate (1mg)
Lane 3: Transfected HEK293 whole cell lysate (10µg)
其他：

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产品描述：

CUSABIO货号：CSB-MA000041M0m Myc标签单克隆抗体是针对Myc表位标签研发的高特异性科研试剂，该抗体采用杂交瘤技术制备，具有批次稳定性和优异的抗原结合能力。Myc标签作为分子生物学研究中广泛应用的短肽标签（EQKLISEEDL），常被用于重组蛋白表达系统的追踪、纯化及功能验证。本产品通过严格验证，适用于Western Blot、免疫荧光、免疫沉淀及ELISA等多种实验体系，可精准识别C端或N端融合的Myc标签蛋白。其广谱物种兼容性覆盖哺乳动物、昆虫及酵母等表达系统，特别适用于重组蛋白表达验证、亚细胞定位分析以及蛋白相互作用研究等实验场景。该抗体经多次亲和纯化处理，在1:1000工作浓度下展现出清晰的检测信号与低背景特性，是蛋白质组学研究和基因功能分析的关键工具试剂。实验方案推荐使用含5%脱脂牛奶的TBST封闭液进行膜封闭，搭配辣根过氧化物酶标记二抗实现可视化检测，助力科研人员高效完成分子互作机制、信号通路解析等前沿课题研究。
别名：

bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64
宿主：

Mouse
反应种属：

N/A
免疫原：

EQKLISEEDL (Myc) synthetic peptide conjugate to KLH
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

IgG1
纯化方式：

>95%, Protein G purified
克隆号：

5F92F9
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品提供形式：

Liquid
应用范围：

ELISA, WB, IF, IP
推荐稀释比：

Application Recommended Dilution

WB 1:500-1:5000

IF 1:100-1:300

IP 1:1000-1:1500
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文献

Borealin-nucleosome interaction secures chromosome association of the chromosomal passenger complex Abad M A, et al,The Journal of Cell Biology,2019
The double-edged sword of endoplasmic reticulum stress in uremic sarcopenia through myogenesis perturbation Jheng JR.et al,J Cachexia Sarcopenia Muscle,2018

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Western blot review for CD146 Monoclonal Antibody

Tested application: WB
Test sample: Cells
Sample volume: 4μg
Electrophoresis parameters: 100V
Primary antibody dilution ratio: 1:500
Review: Antibody specificity is very good.
Western blot review for CD146 Monoclonal Antibody

Tested application: WB
Test sample: HEK293 cell lysate
Sample volume: 20μg
Electrophoresis parameters: 80V/100V
Primary antibody dilution ratio: 1:1000
Review: Myc antibody has good specificity.

Myc tag Monoclonal Antibody

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Application	Recommended Dilution
WB	1:500-1:5000
IF	1:100-1:300
IP	1:1000-1:1500