His-Tag Monoclonal Antibody

中文名称：

His-Tag鼠单克隆抗体
货号：

CSB-MA000159
规格：

￥1090
图片：
IP antibody use: 5µg His Mouse IgG1 per ml Lysate, WB 1:3000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with His-tag fusion protein
Lane 3: IP (untransfected 293 + anti-His mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-His mAb + Protein G agarose)
Lane 6: Recombinant protein (E.coli)

IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)

2µg His fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000
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产品描述：

CUSABIO货号：CSB-MA000159 His-Tag单克隆抗体是一款高特异性科研工具，专为检测带有多组氨酸标签（His-Tag）的重组蛋白设计。该抗体采用杂交瘤技术制备，可精准识别6×His、10×His等常见组氨酸标签序列，适用于所有物种来源的His标签蛋白检测，在Western Blot实验中能清晰呈现目标条带，免疫荧光检测中可实现精准定位，同时支持免疫沉淀技术用于蛋白互作研究及ELISA定量分析。其广谱反应性特点使其成为重组蛋白表达验证、纯化工艺优化及功能研究中的核心试剂，尤其适用于大肠杆菌、哺乳动物细胞等表达系统的蛋白检测。产品经严格质控确保批次稳定性，满足多场景科研需求，为蛋白质组学、分子互作研究及生物制药开发提供可靠支持。关键词：His-Tag单克隆抗体、重组蛋白检测、Western Blot抗体、免疫沉淀试剂、组氨酸标签检测。
宿主：

Mouse
反应种属：

N/A
免疫原：

Synthetic Peptide
标记方式：

Non-conjugated
抗体亚型：

IgG
克隆号：

8F7
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
产品提供形式：

Liquid
应用范围：

ELISA, WB, IF, IP
推荐稀释比：

Application Recommended Dilution

WB 1:500-1:5000

IF 1:50-1:200

IP 1:200-1:2000
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文献

Shank3 ameliorates neuronal injury after cerebral ischemia/reperfusion via inhibiting oxidative stress and inflammation H Zhang,Redox biology,2024
When spider and snake get along: Fusion of a snake disintegrin with a spider phospholipase D to explore their synergistic effects on a tumor cell Siqueira RAGB, et al,Toxicon,2019
Subfunctionalization of the Ruby2-Ruby1 gene cluster during the domestication of citrus Huang D, et al,Nature Plants,2018

His-Tag Monoclonal Antibody

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Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200
IP	1:200-1:2000