HA-Tag Monoclonal Antibody

中文名称：

HA-Tag鼠单克隆抗体
货号：

CSB-MA000158
规格：

￥1090
图片：
IP antibody use: 5µg HA Mouse IgG1 per ml Lysate, WB 1:5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with HA-tag fusion protein
Lane 3: IP (untransfted 293 + anti-HA mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-HA mAb + Protein G agarose)
Lane 6: IP (transfected 293 + Protein G)
Lane 7: Recombinant protein (E.coli)

IF analysis of 293 cells transfected with a HA-tag protein,1:2000 dilution (blue DAPI, red anti-HA)

0.5µg HA fusion protein + Primary antibody dilution at 1:10000
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产品描述：

本产品为高特异性小鼠源HA-Tag单克隆抗体（CUSABIO货号：CSB-MA000158），针对来源于流感病毒血凝素（HA）的表位标签设计，广泛应用于分子生物学研究中融合蛋白的追踪与检测。该抗体经亲和层析纯化制备，可识别重组蛋白中HA标签的YPYDVPDYA线性表位，在Western Blot实验中能有效检测10-150 kDa范围内的标记蛋白，免疫荧光实验适用细胞样本的定位观察，免疫沉淀实验可特异性富集目标蛋白复合物。经严格验证适用于ELISA、WB、IF、IP等实验体系，其广泛物种兼容性支持哺乳动物、昆虫等多种表达系统的HA标签蛋白检测。科研人员可将其应用于基因表达调控研究、蛋白互作网络解析、重组蛋白表达验证等基础研究领域，尤其适合需要高灵敏度检测的转染细胞或原核表达系统样本。
宿主：

Mouse
反应种属：

N/A
免疫原：

Synthetic Peptide
标记方式：

Non-conjugated
抗体亚型：

IgG
克隆号：

26E2
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
产品提供形式：

Liquid
应用范围：

ELISA, WB, IF, IP
推荐稀释比：

Application Recommended Dilution

WB 1:500-1:5000

IF 1:50-1:200

IP 1:200-1:2000
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

HA-Tag Monoclonal Antibody

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Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200
IP	1:200-1:2000