6*His Monoclonal Antibody

中文名称：

6*His鼠单克隆抗体
货号：

CSB-MA000011M0m
规格：

￥400
图片：
Western Blot
Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng
All lanes: HIS antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa

Western Blot
Positive WB detected in: HIS fusion protein 2 at 20ng, 10ng, 5ng, 2.5ng
All lanes: HIS antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
其他：

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产品描述：

CUSABIO货号：CSB-MA000011M0m 6*His单克隆抗体是针对组氨酸标签（6*His）研发的高特异性检测试剂，广泛适用于His标签重组蛋白的检测与分析。该抗体采用杂交瘤技术制备，来源于小鼠免疫球蛋白G，具有高度均一性和特异性，可精准识别重组蛋白中融合表达的6*His标签结构。经严格验证，该产品在Western Blot（WB）和酶联免疫吸附试验（ELISA）中均表现出优异的检测灵敏度，支持跨物种样本检测，适用于大肠杆菌、哺乳动物细胞等多种表达系统中重组蛋白的表达验证、纯化监测及定量分析。在重组蛋白纯化工艺开发、蛋白互作研究、基因工程药物研发等科研领域有重要应用价值，特别适用于需要高特异性识别His标签的蛋白质组学研究。本产品通过严格的质控体系确保批次稳定性，为His标签融合蛋白的检测提供可靠解决方案。
别名：

6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
宿主：

Mouse
反应种属：

N/A
免疫原：

HHHHHH(6x His) synthetic peptide conjugate to KLH
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

IgG2a
纯化方式：

>95%, Protein A purified
克隆号：

3G5H8
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品提供形式：

Liquid
应用范围：

ELISA, WB
推荐稀释比：

Application Recommended Dilution

WB 1:500-1:2000
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文献

cDNA cloning, prokaryotic expression, and functional analysis of squalene synthase (SQS) in Camellia vietnamensis Huang Jiani Dai,Protein Expression and Purification,2023
Lsr2 acts as a cyclic di-GMP receptor that promotes keto-mycolic acid synthesis and biofilm formation in mycobacteria W Wang,Nature communications,2024
Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain Dominguez G.et al,Acta Neuropathol Commun,2018

6*His Monoclonal Antibody

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