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  4. Recombinant Xenopus laevis Zinc finger protein 346 (znf346), partial

Recombinant Xenopus laevis Zinc finger protein 346 (znf346), partial

  • 中文名称：
    有爪蟾蜍znf346重组蛋白
  • 货号：
    CSB-YP026691XBE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    有爪蟾蜍znf346重组蛋白
  • 货号：
    CSB-EP026691XBE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    有爪蟾蜍znf346重组蛋白
  • 货号：
    CSB-EP026691XBE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    有爪蟾蜍znf346重组蛋白
  • 货号：
    CSB-BP026691XBE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    有爪蟾蜍znf346重组蛋白
  • 货号：
    CSB-MP026691XBE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    znf346
  • Uniprot No.：
    Q8AVN9
  • 别名：
    znf346; jazZinc finger protein 346; Double-stranded RNA-binding protein ZFa; DsRBP-ZFa; ZFa; Just another zinc finger protein; Protein jaz
  • 种属：
    Xenopus laevis (African clawed frog)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

We are interested in the product Recombinant Xenopus laevis Zinc finger protein 346 (znf346), CSB-EP026691XBE (E.coli). However we have not decided to purchase the specific tag protein. Appreciate if you could provide us the following information:
1.We would need to conjugate the protein with enzyme, and there are many tag available for the protein. Could you provide me which tag and in which terminal would be appropriate for the purpose (conjugation with enzyme) with good efficiency?
2. Is chemical like SATP and sulfo-SMCC required for the conjugation? or if any other suggested conjugation protocol is available?
3. Also, is the protein expressed in full sequence of partial?

A:
Thanks for your inquiry. Proteins of this species haven't been expressed a lot in expression systems before, but there is a literature reports here:https://www.ncbi.nlm.nih.gov/pubmed/16051273 , this is a double-strand RNA combined protein （The zinc-finger domains are required for binding to dsRNA), and each zinc-finger is connected through linker, the first or the last zinc-finger all has a piece sequence which won't influence whether you add the tag at its N-terminus or C-terminus, but for the structure of the protein begins from the C-terminus, and the customized protein we provide is a fragment at N-terminus, so here we suggest to have a try with the protein which tag is in N-terminus;
What's more, we couldn't grantee its 100% bioactivity for it's a customized protein and we haven't done the related experiments to test its bioactivity. Here is a reference for you:http://www.ebi.ac.uk/pdbe/entry/pdb/1ZU1 , which shows the sequence of the protein at N-terminus can be used in NMR test, and the sequence of Recombinant Xenopus laevis Zinc finger protein 346 (znf346) also is the fragment at N-terminus.
Q:

Could provide me the pI value for the product Recombinant Xenopus laevis Zinc finger protein 346 (znf346) CSB-EP026691XBE?

A:
Thanks for your inquiry. As to your inquiry about the pI value for the product, the predicted theoretical electrical points of this full length protein is 8.07.
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靶点详情

  • 功能：
    Binds preferentially to dsRNA, but also to RNA-DNA hybrids.
  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 数据库链接：

    KEGG: xla:380471

    UniGene: Xl.386

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