Recombinant Xenopus laevis Polyribonucleotide 5-hydroxyl-kinase Clp1 (clp1)

^{In Stock}

货号：

CSB-EP740650XBE
规格：

￥2328
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 85% as determined by SDS-PAGE.
基因名：

clp1
Uniprot No.：

Q6NS21
别名：

Polyadenylation factor Clp1;Polynucleotide kinase Clp1;Pre-mRNA cleavage complex II protein Clp1
种属：

Xenopus laevis (African clawed frog)
蛋白长度：

Full Length
来源：

E.coli
分子量：

54.3 kDa
表达区域：

1-439aa
氨基酸序列

MSSEDLSAPGPSAPDSSWSSTGPSTKFELERETELRLEVEGTDPVRVELVSGLAEVFGTELTRNKKYTFPPGSRAAIFTWHGCTVQLWGSPDMAYVSRDTPMLLYLNTQVGLEQMRVQAEREGERGPRVLVAGPSDVGKSTLCRLLLNYAVRRGRRPTLVELDVGQGSVSVPGTMGALCVERPADVEEGFSAQAPLVYHFGSTTPGTNIKLYNKLTSRLAHVFNLRCDSNRRASVSGCLINTCGWVKGSGYQALIHAASAFEVDVVLVLDQERLYNDLLRDLPHFVRTLLLPKSGGASERSKECRRESRDQRVREYFYGPRGSLYPHAFEIKFSEVRVYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMAHHLLSVVPLDGGSAEEGIEERSVAGFIVITGVDTERQTLTLLSPAPRPLPKCVLLIMDIRFMDLK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal 6xHis-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

3-7 business days
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by dicer1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
亚细胞定位：

Nucleus.
蛋白家族：

Clp1 family, Clp1 subfamily
数据库链接：

KEGG: xla:431824

UniGene: Xl.30457

Recombinant Xenopus laevis Polyribonucleotide 5-hydroxyl-kinase Clp1 (clp1)

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