Recombinant Xenopus laevis E3 ubiquitin-protein ligase RNF12-A (rnf12-a), partial

中文名称：

有爪蟾蜍rnf12-a重组蛋白
货号：

CSB-YP737339XBE
规格：
来源：

Yeast
其他：

中文名称：

有爪蟾蜍rnf12-a重组蛋白
货号：

CSB-EP737339XBE
规格：
来源：

E.coli
其他：

中文名称：

有爪蟾蜍rnf12-a重组蛋白
货号：

CSB-EP737339XBE-B
规格：
来源：

E.coli
共轭：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名称：

有爪蟾蜍rnf12-a重组蛋白
货号：

CSB-BP737339XBE
规格：
来源：

Baculovirus
其他：

中文名称：

有爪蟾蜍rnf12-a重组蛋白
货号：

CSB-MP737339XBE
规格：
来源：

Mammalian cell
其他：

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纯度：

>85% (SDS-PAGE)
基因名：

rnf12-a
Uniprot No.：

Q641J8
别名：

rnf12-a; rnf12; E3 ubiquitin-protein ligase RNF12-A; EC 2.3.2.27; RING finger protein 12-A; RING-type E3 ubiquitin transferase RNF12-A; XRnf12
种属：

Xenopus laevis (African clawed frog)
蛋白长度：

Partial
蛋白标签：

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
产品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

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功能：

Acts as an E3 ubiquitin-protein ligase specific for ldb1, mediating ubiquitination and proteasome-dependent degradation of excess ldb1 in a RING-dependent manner. Does not degrade ldb1 bound to lhx1/lim1, nor lim1 itself and thus contributes to the establishment of proper ldb1-lhx1/lim1 stoichiometry and the formation of a ldb1-lhx1/lim1 complex. Interferes with Spemann organizer function and suppresses secondary axis formation induced by ldb1 and lhx1/lim1.
亚细胞定位：

Nucleus.
蛋白家族：

RNF12 family
组织特异性：

Shows overlapping expression with lhx1/lim1 and ldb1 in the gastrula mesoderm, and expression overlaps with ldb1 throughout early embryogenesis. After gastrulation, expression is gradually restricted to tissues originated from the ectoderm, the neuroectod
数据库链接：

KEGG: xla:398680

UniGene: Xl.11908

Recombinant Xenopus laevis E3 ubiquitin-protein ligase RNF12-A (rnf12-a), partial

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