Recombinant Vesicular stomatitis Indiana virus Matrix protein (M)

^{In Stock}

货号：

CSB-EP356070VBJ
规格：

￥2328
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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纯度：

Greater than 85% as determined by SDS-PAGE.
基因名：

M
Uniprot No.：

P03519
种属：

Vesicular stomatitis Indiana virus (strain San Juan) (VSIV)
蛋白长度：

Full Length
来源：

E.coli
分子量：

33.5 kDa
表达区域：

1-229aa
氨基酸序列

MSSLKKILGLKGKGKKSKKLGIAPPPYEEDTSMEYAPSAPIDKSYFGVDEMDTYDPNQLRYEKFFFTVKMTVRSNRPFRTYSDVAAAVSHWDHMYIGMAGKRPFYKILAFLGSSNLKATPAVLADQGQPEYHTHCEGRAYLPHRMGKTPPMLNVPEHFRRPFNIGLYKGTIELTMTIYDDESLEAAPMIWDHFNSSKFSDFREKALMFGLIVEKKASGAWVLDSISHFK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

3-7 business days
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Plays a major role in assembly and budding of virion, by recruiting cellular partners of the ESCRT complexes that play a key role in releasing the budding particle from the host membrane. Condensates the ribonucleocapsid core during virus assembly.; Inhibits mRNA nuclear export through direct interaction with host RAE1-NUP98 complex, thereby preventing interferon signaling and establishment of antiviral state in infected cells. Induces cell-rounding, cytoskeleton disorganization and apoptosis in infected cell. Inhibits host transcription, possibly through interaction with host DNA repair factor IIH/TFIIH GTF2H5 subunit.
基因功能参考文献：
1. VEGF-D-enhanced metastasis was evidently reversed by MP. MP significantly reduced the invasion of VEGFD-SK cells, tumor volume, lymphatic metastasis rates and lymphatic vessel density compared with control groups PMID: 27211072
2. vesicular stomatitis virus M protein interacted efficiently with Rae1-Nup98 complexes associated with the chromatin fraction of host nuclei, consistent with an effect on host transcription PMID: 23028327
3. These findings indicate that the PSAP motif plays a direct role in regulating cytopathogenicity in a species-dependent manner, and suggest that the intact PSAP motif may be important for maintaining virus persistence in an insect host. PMID: 23170175
4. study re-examined the contribution of the PSAP motif to VSV budding; VSV M binds TSG101 [tumour susceptibility gene 101] through its PSAP motif; double mutant with point mutations in both PSAP and PPPY motifs is impaired compared with a single mutant in the PPPY motif, indicating the PSAP motif partially compensates for lack of the PPPY motif PMID: 22190013
5. VSV M protein inactivates the cellular Akt signaling pathway. PMID: 20980511
6. These findings indicate that the host shut-off activity of the M protein dominates vesicular stomatitis virus cytotoxicty, whilst the fusion-active G protein is mainly responsible for the cytotoxicity remaining with M-mutant vesicular stomatitis virus. PMID: 20631091
7. hyperphosphorylation of VSV M protein was observed in both cell lysates and viral particles from IFN-beta-treated neuronal cells. Hyperphosphorylated VSV M protein was found to inhibit its association with VSV nucleocapsid PMID: 19951173
8. VSV expressing the mutant M protein induces apoptosis via the death receptor apoptotic pathway, a mechanism distinct from that induced by VSV expressing the wt M protein. PMID: 15767418
9. first evidence that M protein alone is able to impose the correct budding curvature on the membrane PMID: 16298982
10. Glycoprotein (G protein) and M protein microdomains were not colocalized in the plasma membrane outside the virus budding sites PMID: 18367537
11. M protein mutants of VSV are less virulent than wild type and can induce an antibody response similar to that in mice infected with the wt virus. PMID: 18614644
12. The authors propose that low-pH conformational changes in G protein promote acidification of the virus interior, which facilitates the release of M from ribonucleoprotein particles during uncoating. PMID: 19776119
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亚细胞定位：

Virion membrane; Peripheral membrane protein. Host endomembrane system; Peripheral membrane protein. Host nucleus membrane; Peripheral membrane protein. Host nucleus. Host cytoplasm.
蛋白家族：

Vesiculoviruses matrix protein family
数据库链接：

KEGG: vg:1489833

Recombinant Vesicular stomatitis Indiana virus Matrix protein (M)

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