Recombinant Thermotoga maritima 5-keto-L-gluconate epimerase (iolO)

中文名称：

海栖热袍菌iolO重组蛋白
货号：

CSB-EP893461TNJ
规格：

￥2328
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 85% as determined by SDS-PAGE.
基因名：

iolO
Uniprot No.：

Q9WYP7
别名：

Bifunctional nonphosphorylated sugar isomerase (D-erythrose/D-threose isomerase) (L-ribulose 3-epimerase) (R3E) (Nonphosphorylated sugar 3-epimerase) (Nonphosphorylated sugar aldose-ketose isomerase)
种属：

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
蛋白长度：

Full Length
来源：

E.coli
分子量：

37.9 kDa
表达区域：

1-270aa
氨基酸序列

MKLSLVISTSDAAFDALAFKGDLRKGMELAKRVGYQAVEIAVRDPSIVDWNEVKILSEELNLPICAIGTGQAYLADGLSLTHPNDEIRKKAIERVVKHTEVAGMFGALVIIGLVRGRREGRSYEETEELFIESMKRLLELTEHAKFVIEPLNRYETDFINTIDDALRILRKINSNRVGILADTFHMNIEEVNIPESLKRAGEKLYHFHVADSNRWAPGCGHFDFRSVFNTLKEIGYNRYVSVECLPLPGGMEEAAEIAFKTLKELIIKLT
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

13-23 business days
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Catalyzes the reversible epimerization between 5-keto-L-gluconate (5-dehydro-L-gluconate) and D-tagaturonate, and thus probably functions in a myo-inositol degradation pathway together with IolG, IolM and IolN. Is not active on the enantiomer 5-keto-D-gluconate. Was also shown to be a nonphosphorylated sugar isomerase with broad substrate specificity in vitro. Is able to catalyze the reversible C3-epimerization of L-ribulose to L-xylulose, D-ribulose to D-xylulose, D-psicose to D-fructose, and D-tagatose to D-sorbose, with a substrate preference for ketopentoses rather than ketohexoses. Also catalyzes the aldose-ketose isomerization reaction from either D-erythrose or D-threose to D-erythrulose. Exhibits no activity for C4-epimerization of D-tagatose to D-fructose.
基因功能参考文献：
1. TM0416 is a putative nonphosphorylated sugar epimerase and catalyzes various C5 and C6 epimerization reactions. PMID: 28258150
蛋白家族：

Hyi family
数据库链接：

KEGG: tma:TM0416

STRING: 243274.TM0416

Recombinant Thermotoga maritima 5-keto-L-gluconate epimerase (iolO)

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