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  4. Recombinant Thermosynechococcus elongatus Circadian clock protein kinase kaiC (kaiC), partial

Recombinant Thermosynechococcus elongatus Circadian clock protein kinase kaiC (kaiC), partial

  • 中文名称：
    热链球菌kaiC重组蛋白
  • 货号：
    CSB-YP762376TDQ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    热链球菌kaiC重组蛋白
  • 货号：
    CSB-EP762376TDQ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    热链球菌kaiC重组蛋白
  • 货号：
    CSB-EP762376TDQ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    热链球菌kaiC重组蛋白
  • 货号：
    CSB-BP762376TDQ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    热链球菌kaiC重组蛋白
  • 货号：
    CSB-MP762376TDQ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    kaiC
  • Uniprot No.：
    Q79V60
  • 别名：
    kaiC; tlr0483Circadian clock protein kinase KaiC; EC 2.7.11.1
  • 种属：
    Thermosynechococcus elongatus (strain BP-1)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Core component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction.
  • 基因功能参考文献：
    1. KaiC crystal structure shows arginine tetrad as mediator of input-dependent and input-independent ATPase activities. PMID: 24816106
    2. ATP regulates formation of the KaiB-KaiC complex and circadian clock in Thermosynechococcus. PMID: 24244649
    3. circadian time information (free phosphorylated SasA) is released mainly by unphosphorylated KaiC during the late subjective night. PMID: 22512339
    4. role of the two sets of ATPase motifs PMID: 15377674
    5. The KaiC hexamer consists of a rigid ring structure formed by six N-terminal domains with hexamerization activity and a flexible structure formed by six C-terminal domains with intersubunit phosphorylation activity. PMID: 16901465
    6. C-terminal phosphorylation sites of a KaiC hexamer subunit are phosphorylated by the C-terminal domain of an adjacent KaiC subunit; phosphorylation suppresses ATPase activity of the C-terminal domain; and phosphorylated KaiC spontaneously dephosphorylates PMID: 18363969

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  • 蛋白家族：
    KaiC family
  • 数据库链接：

    KEGG: tel:tlr0483

    STRING: 197221.tlr0483

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