Recombinant Streptococcus pneumoniae Penicillin-binding protein 2X (pbpX), partial,Biotinylated

中文名称：

肺炎链球菌pbpX重组蛋白
货号：

CSB-EP348688SMP-B
规格：

￥3324
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 85% as determined by SDS-PAGE.
基因名：

pbpX
Uniprot No.：

P59676
种属：

Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
蛋白长度：

Partial
来源：

E.coli
分子量：

83.2 kDa
表达区域：

287-611aa
氨基酸序列

KYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYEPGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGFAHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSFGQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAASLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLTDYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins.
基因功能参考文献：
1. Kinetic Evidence for a Second Ligand Binding Site on Streptococcus pneumoniae Penicillin-Binding Protein 2x. PMID: 29485264
2. The mutation Ala707Asp within the C-terminal PASTA2 domain leads to protein destabilization. PMID: 24841912
3. Depletion of PBP2x resulted in severe morphological alterations, confirming the essentiality of PBP2x and demonstrating that PBP2x is required for cell division and not for cell elongation. PMID: 24655324
4. Mutations of a PBP relevant to beta-lactam resistance have been identified, providing new mechanistic insights. PMID: 16303769
5. pbp2x gene was detected in all the resistant strains and that is why we could conclude that it was coding the high level of resistance. PMID: 19736537
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亚细胞定位：

Cell membrane; Single-pass membrane protein.
蛋白家族：

Transpeptidase family
数据库链接：

KEGG: spr:spr0304

STRING: 171101.spr0304

Recombinant Streptococcus pneumoniae Penicillin-binding protein 2X (pbpX), partial,Biotinylated

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