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  4. Recombinant Schizosaccharomyces pombe Serine/threonine-protein kinase ppk4 (ppk4), partial

Recombinant Schizosaccharomyces pombe Serine/threonine-protein kinase ppk4 (ppk4), partial

  • 中文名称：
    庞贝裂殖酵母ppk4重组蛋白
  • 货号：
    CSB-YP529692SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    庞贝裂殖酵母ppk4重组蛋白
  • 货号：
    CSB-EP529692SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    庞贝裂殖酵母ppk4重组蛋白
  • 货号：
    CSB-EP529692SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    庞贝裂殖酵母ppk4重组蛋白
  • 货号：
    CSB-BP529692SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    庞贝裂殖酵母ppk4重组蛋白
  • 货号：
    CSB-MP529692SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ppk4
  • Uniprot No.：
    O94537
  • 别名：
    ppk4; SPAC167.01; Serine/threonine-protein kinase ppk4; EC 2.7.11.1
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endoplasmic reticulum (ER) membrane-resident kinase/endoribonuclease involved in unfolded protein response (UPR). Initiates the selective decay of a subset of ER-localized-mRNAs that is required to survive ER stress. Rather than relying on a transcriptional program to up-regulate genes that enhance ER protein folding capacity as in S.cerevisiae, S.pombe cells reduce the amount of specific proteins entering the organelle by decreasing the level of ER-targeted mRNAs using ire1-dependent mRNA degradation. The sole mRNA cleaved upon ire1 activation that escapes decay is the ER chaperone bip1 mRNA which is more stable and hence is present at an increased steady-state after ire1 cleavage. Promotes ER stress-induced ER-phagy and via up-regulation of the protein level of the ER-phagy receptor epr1 (Ref.5).
  • 基因功能参考文献：
    1. These results reveal that the unfolded protein response in Schizosaccharomyces pombe executes selective degradation of endoplasmic reticulum-bound mRNAs in an intricate interplay between Ire1, translation, and the mRNA no-go-decay pathway, and establish a critical role for mRNA no-go-decay in maintaining endoplasmic reticulum homeostasis. PMID: 28945192
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 数据库链接：

    KEGG: spo:SPAC167.01

    STRING: 4896.SPAC167.01.1

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