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  4. Recombinant Schizosaccharomyces pombe Kinesin-like protein cut7 (cut7), partial

Recombinant Schizosaccharomyces pombe Kinesin-like protein cut7 (cut7), partial

  • 中文名称：
    Recombinant Schizosaccharomyces pombe Kinesin-like protein cut7(cut7) ,partial
  • 货号：
    CSB-YP328463SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Schizosaccharomyces pombe Kinesin-like protein cut7(cut7) ,partial
  • 货号：
    CSB-EP328463SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Schizosaccharomyces pombe Kinesin-like protein cut7(cut7) ,partial
  • 货号：
    CSB-EP328463SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Schizosaccharomyces pombe Kinesin-like protein cut7(cut7) ,partial
  • 货号：
    CSB-BP328463SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Schizosaccharomyces pombe Kinesin-like protein cut7(cut7) ,partial
  • 货号：
    CSB-MP328463SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    cut7
  • Uniprot No.：
    P24339
  • 别名：
    cut7; SPAC25G10.07cKinesin-like protein cut7; Cell untimely torn protein 7
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Could be a spindle pole body motor. On transition from G2 to M phase of the cell cycle, the spindle pole body duplicates; the daughter pole bodies seed microtubules which interdigitate to form a short spindle that elongates to span the nucleus at metaphase. Mutations at cut7 block spindle formation.
  • 基因功能参考文献：
    1. The study shows that the stepping direction of Cut7, a yeast kinesin-5 motor, depends on the level of motor crowding on the microtubule, and that crowding of Cut7 by non-Cut7 proteins also can drive reversal. Thus, crowding by either dynein microtubule binding domain or Klp2, a kinesin-14, converts Cut7 from net minus end-directed to net plus end-directed stepping. PMID: 27834216
    2. Molecular properties of the N-terminal extension of the fission yeast kinesin-5, Cut7, have been reported. PMID: 26909973
    3. In in vitro motility assays, full-length Cut7 moved toward minus-end of microtubules, but the N-terminal half of Cut7 moved toward the opposite direction. PMID: 24589736
  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, BimC subfamily
  • 数据库链接：

    KEGG: spo:SPAC25G10.07c

    STRING: 4896.SPAC25G10.07c.1

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