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  4. Recombinant Schizosaccharomyces pombe Inositol-1,4,5-trisphosphate 5-phosphatase 1 (syj1), partial

Recombinant Schizosaccharomyces pombe Inositol-1,4,5-trisphosphate 5-phosphatase 1 (syj1), partial

  • 中文名称：
    庞贝裂殖酵母syj1重组蛋白
  • 货号：
    CSB-YP523671SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    庞贝裂殖酵母syj1重组蛋白
  • 货号：
    CSB-EP523671SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    庞贝裂殖酵母syj1重组蛋白
  • 货号：
    CSB-EP523671SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    庞贝裂殖酵母syj1重组蛋白
  • 货号：
    CSB-BP523671SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    庞贝裂殖酵母syj1重组蛋白
  • 货号：
    CSB-MP523671SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    syj1
  • Uniprot No.：
    O43001
  • 别名：
    syj1; SPBC2G2.02; Inositol-1,4,5-trisphosphate 5-phosphatase 1; EC 3.1.3.36; Synaptojanin-like protein 1
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Controls the cellular levels and subcellular distribution of phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-bisphosphate. Involved in distinct membrane trafficking and signal transduction pathways. Highly active against a range of soluble and lipid inositol phosphates. Active in dephosphorylating the 5-position of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 and to a lesser extent Ins(1,4,5,6)P4. The enzyme is also active against PI(4,5)P2 presented in sonicated vesicles and Triton mixed micelles, and somewhat less active against PI(3,5)P2 in unilamellar vesicles. Activity against PI(3,5)P2 drops sharply when this substrate is presented in mixed micelles. Also hydrolyzes PIP3 to produce PI(3,4)P2.
  • 基因功能参考文献：
    1. Schizosaccharomyces pombe synaptojanin (SPsynaptojanin) and human SH2 domain-containing inositol-5-phosphatase SHIP2 comparative study: mechanism and substrate specificity. [SPsynaptojanin] PMID: 15316017
    2. Reports the crystal structure of the catalytic domain of S. pombe synaptojanin (SPsynaptojanin) alone at 2.0 angstrom resolution, and in a product complex with inositol (1,4)-bisphosphate and calcium at 1.8 angstrom resolution. PMID: 11348594
  • 亚细胞定位：
    Cytoplasm. Note=Localizes at the cell tip and the barrier septum.
  • 蛋白家族：
    Synaptojanin family; Inositol 1,4,5-trisphosphate 5-phosphatase family
  • 数据库链接：

    KEGG: spo:SPBC2G2.02

    STRING: 4896.SPBC2G2.02.1

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