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  4. Recombinant Schizosaccharomyces pombe Importin subunit beta-3 (sal3), partial

Recombinant Schizosaccharomyces pombe Importin subunit beta-3 (sal3), partial

  • 中文名称：
    庞贝裂殖酵母sal3重组蛋白
  • 货号：
    CSB-YP529184SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    庞贝裂殖酵母sal3重组蛋白
  • 货号：
    CSB-EP529184SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    庞贝裂殖酵母sal3重组蛋白
  • 货号：
    CSB-EP529184SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    庞贝裂殖酵母sal3重组蛋白
  • 货号：
    CSB-BP529184SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    庞贝裂殖酵母sal3重组蛋白
  • 货号：
    CSB-MP529184SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    sal3
  • Uniprot No.：
    O74476
  • 别名：
    sal3; pse1; SPCC1840.03; Importin subunit beta-3; Importin beta sal3
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the nuclear import of cdc25 and mcs1.; Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Involved in the nuclear import of cdc25 and mcs1. Mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to repeat-containing nucleoporins. The complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, GTP-Ran binding leads to release of the cargo. The importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • 基因功能参考文献：
    1. Further analysis by pull-down experiments revealed that Php4 is a cargo of the karyopherins Imp1, Cut15 and Sal3. PMID: 25330182
    2. The karyopherin Sal3 is required for nuclear import of Rdp1 PMID: 22268381
  • 亚细胞定位：
    Cytoplasm. Nucleus envelope. Nucleus.
  • 蛋白家族：
    Importin beta family, Importin beta-3 subfamily
  • 数据库链接：

    KEGG: spo:SPCC1840.03

    STRING: 4896.SPCC1840.03.1

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