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  4. Recombinant Schizosaccharomyces pombe Dual specificity protein kinase pom1 (pom1), partial

Recombinant Schizosaccharomyces pombe Dual specificity protein kinase pom1 (pom1), partial

  • 中文名称：
    庞贝裂殖酵母pom1重组蛋白
  • 货号：
    CSB-YP600648SXV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    庞贝裂殖酵母pom1重组蛋白
  • 货号：
    CSB-EP600648SXV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    庞贝裂殖酵母pom1重组蛋白
  • 货号：
    CSB-EP600648SXV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    庞贝裂殖酵母pom1重组蛋白
  • 货号：
    CSB-BP600648SXV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    庞贝裂殖酵母pom1重组蛋白
  • 货号：
    CSB-MP600648SXV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    pom1
  • Uniprot No.：
    Q09690
  • 别名：
    pom1; SPAC2F7.03c; DYRK-family kinase pom1; EC 2.7.12.1
  • 种属：
    Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Polarity factor involved in localization of polarized growth and cytokinesis. Forms an intracellular gradient that serves to measure cell length and control mitotic entry. Controls the timing of mitotic commitment by regulating the inhibitory impact of cdr1/cdr2 on wee1 activity. Directly phosphorylates the tail of cdr2 which inhibits cdr2 activation by ssp1. Cdr2 phosphorylation by pom1 also modulates cdr2 association with membranes and inhibits cdr2 interaction with mid1, reducing its clustering ability, possibly via the down-regulation of cdr2 kinase activity. Acts as a negative regulator of mid1 distribution, excluding mid1 from non-growing ends, which prevents division-septum assembly at the cell ends. The pom1 polar gradient also mediates mitotic entry by regulating cdk1. Plays an essential role in proper localization and phosphorylation of a GAP for cdc42, rga4, which ensures bipolar localization of GTP-bound, active cdc42 involved in F-actin formation. Phosphorylates multiple other substrates that function in polarized cell growth, including tea4, mod5, pal1, the Rho GAP rga7, and the Arf GEF syt22.
  • 基因功能参考文献：
    1. Results demonstrate that Pom1 auto-phosphorylates intermolecularly, both in vitro and in vivo, which confers robustness to the gradient. PMID: 26150232
    2. Cells deleted for pom1 have a greater size distribution at mitosis compared with wild-type, which could suggest a role for Pom1 as a cell size sensor. PMID: 24047646
    3. these data lead to a two-state model for gradient formation in which pom1p molecules associate with the plasma membrane at cell tips and then diffuse on the membrane while aggregating into and fragmenting from clusters, before disassociating from the membrane. PMID: 22342545
    4. Study shows that Tea4, which is normally deposited at cell tips by microtubules, is both necessary and, upon ectopic cortical localization, sufficient to recruit Pom1 to the cell cortex. PMID: 21703453
    5. Pom1 kinase acts as a negative regulator of Mid1p distribution, excluding Mid1p from non-growing ends, whereas a Pom1-independent mechanism prevents Mid1p association with growing ends. PMID: 17077120
    6. Study provides evidence that pom1p, a DYRK-family protein kinase that forms a concentration gradient emanating from the nongrowing cell end, inhibits mid1p, in pom1 mutants, mid1p is distributed over half of the cell, covering the nongrowing cell end. PMID: 17140794
    7. the cell polarity protein kinase Pom1, which localizes to cell ends, regulates a signalling network that contributes to the control of mitotic entry PMID: 19474789
    8. an intracellular gradient of the dual-specificity tyrosine-phosphorylation regulated kinase (DYRK) Pom1, which emanates from the ends of rod-shaped Schizosaccharomyces pombe cells, serves to measure cell length and control mitotic entry PMID: 19474792

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  • 亚细胞定位：
    Cell tip. Cell septum. Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MNB/DYRK subfamily
  • 数据库链接：

    KEGG: spo:SPAC2F7.03c

    STRING: 4896.SPAC2F7.03c.1

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