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  4. Recombinant Saccharomyces cerevisiae V-type proton ATPase subunit a, vacuolar isoform (VPH1), partial

Recombinant Saccharomyces cerevisiae V-type proton ATPase subunit a, vacuolar isoform (VPH1), partial

  • 中文名称：
    酿酒酵母VPH1重组蛋白
  • 货号：
    CSB-YP339276SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母VPH1重组蛋白
  • 货号：
    CSB-EP339276SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母VPH1重组蛋白
  • 货号：
    CSB-EP339276SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母VPH1重组蛋白
  • 货号：
    CSB-BP339276SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母VPH1重组蛋白
  • 货号：
    CSB-MP339276SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    VPH1
  • Uniprot No.：
    P32563
  • 别名：
    V-ATPase 95 kDa subunit; V-ATPase a 1 subunit; V-type proton ATPase subunit a; vacuolar isoform; Vacuolar pH protein 1; Vacuolar proton pump a subunit; Vacuolar proton translocating ATPase subunit a 1; vph1; VPH1_YEAST
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Subunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in vacuolar V-ATPase complexes. Enzymes containing this subunit have a 4-fold higher ratio of proton transport to ATP hydrolysis than complexes containing the Golgi/endosomal isoform and undergo reversible dissociation of V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
  • 基因功能参考文献：
    1. we show that the role of Sch9 in regulating ageing is tightly connected with V-ATPase activity and vacuolar acidity. As both Sch9 and the V-ATPase are highly conserved in higher eukaryotes, it will be interesting to further clarify their cooperative action on the cellular processes that influence growth and ageing. PMID: 28604780
    2. Inhibitors of V-ATPase proton transport reveal uncoupling functions of tether linking cytosolic and membrane domains of V0 subunit a (Vph1p). PMID: 22215674
    3. Consequences of loss of Vph1 protein-containing vacuolar ATPases (V-ATPases) for overall cellular pH homeostasis. PMID: 21669878
    4. the large flexibility of R735 might be important for its function in proton translocation in the V-ATPase enzyme PMID: 16257593
    5. osteopetrotic a3 mutations recreated in yeast retain 65-70% of activity with wild type expression of V-ATPase subunits; human a4 dRTA mutation G820R recreated in yeast resulted in inactive but assembled complex in the vacuole membrane PMID: 16840787
    6. Membrane-bound peptides from V-ATPase subunit a do not interact with an indole-type inhibitor. PMID: 18069732

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  • 亚细胞定位：
    Vacuole membrane; Multi-pass membrane protein.
  • 蛋白家族：
    V-ATPase 116 kDa subunit family
  • 数据库链接：

    KEGG: sce:YOR270C

    STRING: 4932.YOR270C

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