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  4. Recombinant Saccharomyces cerevisiae ATP-dependent helicase FUN30 (FUN30), partial

Recombinant Saccharomyces cerevisiae ATP-dependent helicase FUN30 (FUN30), partial

  • 中文名称：
    酿酒酵母FUN30重组蛋白
  • 货号：
    CSB-YP330077SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母FUN30重组蛋白
  • 货号：
    CSB-EP330077SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母FUN30重组蛋白
  • 货号：
    CSB-EP330077SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母FUN30重组蛋白
  • 货号：
    CSB-BP330077SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母FUN30重组蛋白
  • 货号：
    CSB-MP330077SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FUN30
  • Uniprot No.：
    P31380
  • 别名：
    FUN30; YAL019W; YAL001ATP-dependent helicase FUN30; EC 3.6.4.12
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs, facilitating single-stranded DNA (ssDNA) production by the EXO1 and SGS1 machinery. Promotes gene silencing at heterochromatin by regulating the chromatin structure within or around silent loci. Also required for heterochromatin organization at centromeres.
  • 基因功能参考文献：
    1. Budding yeast Fun30 and human SMARCAD1 are cell cycle-regulated by interaction with the DNA double strand break-localized scaffold protein Dpb11/TOPBP1, respectively. In yeast, this protein assembly additionally comprises the 9-1-1 damage sensor, is involved in localizing Fun30 to damaged chromatin, and thus is required for efficient long-range resection of DNA double strand breaks. PMID: 28063255
    2. Fun30 acts to down regulate Rad9-dependent DNA damage checkpoint triggered by CPT or MMS, but does not affect Rad9-independent intra-S phase replication checkpoint induced by MMS or HU. It supports the notion that Fun30 contributes to cellular response to DSBs by preventing excessive DNA damage checkpoint activation in addition to its role in facilitating DNA end resection. PMID: 28089177
    3. Cdk1 and multiple cyclins become highly enriched at DSBs and that the recruitment of Cdk1 and cyclins Clb2 and Clb5 ensures optimal Fun30 phosphorylation and checkpoint activation. PMID: 26801641
    4. These results define a role for Fun30 in the regulation of transcription and indicate that Fun30 remodels chromatin at the 5' end of genes by sliding promoter-proximal nucleosomes. PMID: 23779104
    5. Fun30, a poorly characterized member of the Swi2/Snf2 family of chromatin remodelers, plays a role in end processing by facilitating the Exo1 and Sgs1-Dna2 resection pathways. PMID: 23147792
    6. Fun30 is required to allow the adaptation of DNA damage checkpoint-arrested cells with an unrepaired double-strand break to resume cell cycle progression. PMID: 23007155
    7. our findings indicate a direct role of Fun30 in centromere chromatin by promoting correct chromatin architecture. PMID: 23028372
    8. the yeast nucleosome-remodelling enzyme Fun30 is a factor promoting double-strand break end resection; data suggest that Fun30 helps to overcome the inhibitory effect of Rad9 on DNA resection PMID: 22960743
    9. findings unveil an evolutionarily conserved role for the Fun30 and SMARCAD1 chromatin remodellers in controlling end resection, homologous recombination and genome stability in the context of chromatin PMID: 22960744
    10. the Snf2 homolog Fun30 acts as a homodimeric ATP-dependent chromatin-remodeling enzyme PMID: 20075079
    11. Fun30 is directly involved in silencing by regulating the chromatin structure within or around silent loci. PMID: 19956593

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  • 亚细胞定位：
    Nucleus. Chromosome. Note=Recruited to double-strand breaks (DSBs) sites of DNA damage.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 数据库链接：

    KEGG: sce:YAL019W

    STRING: 4932.YAL019W

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