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  4. Recombinant Saccharomyces cerevisiae Transcriptional regulatory protein GAT1 (GAT1), partial

Recombinant Saccharomyces cerevisiae Transcriptional regulatory protein GAT1 (GAT1), partial

  • 中文名称：
    酿酒酵母GAT1重组蛋白
  • 货号：
    CSB-YP331873SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母GAT1重组蛋白
  • 货号：
    CSB-EP331873SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母GAT1重组蛋白
  • 货号：
    CSB-EP331873SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母GAT1重组蛋白
  • 货号：
    CSB-BP331873SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母GAT1重组蛋白
  • 货号：
    CSB-MP331873SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GAT1
  • Uniprot No.：
    P43574
  • 别名：
    GAT1; YFL021W; Transcriptional regulatory protein GAT1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Positive regulator of multiple nitrogen catabolic genes.
  • 基因功能参考文献：
    1. Based on these results, the genetic engineering on Gat1p has a great potential in enhancing non-preferred nitrogen metabolism in S. cerevisiae. PMID: 26899143
    2. Data show that the GAT1 gene, encoding a transcriptional activator of nitrogen-responsive catabolic genes, produces a variety of mRNAs differing in their 5' and 3' termini. PMID: 26259534
    3. We demonstrate that wild type Gat1 isoforms (IsoA and IsoB) are initiated at Gat1 methionines 40, 95, and/or 102, but not at methionine 1 regardless of nitrogen conditions. PMID: 24324255
    4. Rapamycin-dependent Gat1 and Gln3 binding to the Aro80 target promoters is not affected by tryptophan availability, suggesting that transactivation activity of Aro80 is not necessary for the recruitment of GATA factors. PMID: 23651256
    5. Gln3, Gat1, and Ure2 respond to TorC1 and nitrogen availability via distinct regulatory pathways. PMID: 23184930
    6. Controlling lipid fluxes at glycerol-3-phosphate acyltransferase step in yeast: unique contribution of Gat1p to oleic acid-induced lipid particle formation. PMID: 22267742
    7. Nitrogen-responsive regulation of GATA protein family activators Gln3 and Gat1 occurs by two distinct pathways, one inhibited by rapamycin and the other by methionine sulfoximine. PMID: 22039046
    8. Gln3 and Gat1 localizations are controlled by two different regulatory pathways PMID: 20378536
    9. role in regulation of invertase activity and SUC2 expression PMID: 15780659
    10. Rtg1/3p, Gln3p, and Gat1p can be differentially regulated through different nutrient-sensing pathways, such as TOR and retrograde signaling, and by multiple factors, such as Lst8p, which is suggested to have a role in connecting the RTG and TOR pathways PMID: 16253991
    11. in the absence of urmylation, nuclear/cytosolic shuffling of both Nil1 and Gln3 transcriptional factors is altered, ultimately leading to inability to repress GAP1 gene PMID: 17254574
    12. Tor pathway control of the nitrogen-responsive DAL5 gene bifurcates at the level of Gln3 and Gat1 regulation in Saccharomyces cerevisiae PMID: 18245087
    13. Results show that Gat1 is a key factor for the integrated control of nitrogen catabolite repression in Saccharomyces cerevisiae. PMID: 19380492
    14. The phosphorylation of Put3p appears to influence the association of Gat1p, but not Gln3p, to the PUT1 promoter. PMID: 19574222

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: sce:YFL021W

    STRING: 4932.YFL021W

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