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  4. Recombinant Saccharomyces cerevisiae Transcription initiation factor IIF subunit beta (TFG2)

Recombinant Saccharomyces cerevisiae Transcription initiation factor IIF subunit beta (TFG2)

  • 中文名称：
    酿酒酵母TFG2重组蛋白
  • 货号：
    CSB-YP340454SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母TFG2重组蛋白
  • 货号：
    CSB-EP340454SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母TFG2重组蛋白
  • 货号：
    CSB-EP340454SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母TFG2重组蛋白
  • 货号：
    CSB-BP340454SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母TFG2重组蛋白
  • 货号：
    CSB-MP340454SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TFG2
  • Uniprot No.：
    P41896
  • 别名：
    TFG2; YGR005C; Transcription initiation factor IIF subunit beta; TFIIF medium subunit; TFIIF-beta; Transcription factor G 54 kDa subunit
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-400
  • 氨基酸序列
    MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHGQ ELGKIRINKD GSKITLLLNE NDNDSIPHEY DLELTKKVVE NEYVFTEQNL KKYQQRKKEL EADPEKQRQA YLKKQEREEE LKKKQQQQKR RNNRKKFNHR VMTDRDGRDR YIPYVKTIPK KTAIVGTVCH ECQVMPSMND PNYHKIVEQR RNIVKLNNKE RITTLDETVG VTMSHTGMSM RSDNSNFLKV GREKAKSNIK SIRMPKKEIL DYLFKLFDEY DYWSLKGLKE RTRQPEAHLK ECLDKVATLV KKGPYAFKYT LRPEYKKLKE EERKATLGEL ADEQTGSAGD NAQGDAEADL EDEIEMEDVV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    TFIIF is a general transcription initiation factor that binds to RNA polymerase II. Its functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation.
  • 基因功能参考文献：
    1. Tfg2 C-terminal domain interacts with double-stranded DNA oligonucleotides. PMID: 22095626
    2. We show that Anc1 is required for growth on galactose as the sole carbon source, and that it is recruited to the UAS of the GAL1 gene after induction. PMID: 15896708
    3. Evidence that the Tfg1/Tfg2 dimer interface of TFIIF is located in proximity to the B-finger of TFIIB near the active center of RNA polymerase II (RNAP II) where the TFIIB-TFIIF-RNAP II interface plays a key role in transcription start site selection. PMID: 16147988
    4. Tfg2p is retained in the nucleus through DNA binding in Saccharomyces cerevisiae. PMID: 19366694
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TFIIF beta subunit family
  • 数据库链接：

    KEGG: sce:YGR005C

    STRING: 4932.YGR005C

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