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  4. Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase TEL1 (TEL1), partial

Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase TEL1 (TEL1), partial

  • 中文名称：
    酿酒酵母TEL1重组蛋白
  • 货号：
    CSB-YP334371SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母TEL1重组蛋白
  • 货号：
    CSB-EP334371SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母TEL1重组蛋白
  • 货号：
    CSB-EP334371SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母TEL1重组蛋白
  • 货号：
    CSB-BP334371SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母TEL1重组蛋白
  • 货号：
    CSB-MP334371SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TEL1
  • Uniprot No.：
    P38110
  • 别名：
    TEL1; YBL088C; YBL0706Serine/threonine-protein kinase TEL1; EC 2.7.11.1; ATM homolog; DNA-damage checkpoint kinase TEL1; Telomere length regulation protein 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Recruited by the MRX-complex to sites of DNA lesions immediately after damage to initiate non-homologous end-joining (NHEJ). Subsequently displaced by the RPA complex in a reaction probably involving the SAE2 protein. Phosphorylates MRE11 and XRS2, 2 subunits of the MRX-complex. The phosphorylation of MRE11 is a feedback response from the checkpoint signaling pathway. Phosphorylates RAD9, CHK1 and RAD53, leading to the activation of the CHK1 and RAD23 kinases involved in the DNA damage response cascade. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, also involved in the regulation of DNA damage response mechanism. Phosphorylates also SLX4 and RTT107 which are involved in genome stability. Required for the control of telomere length and genome stability.
  • 基因功能参考文献：
    1. Our findings provide a model in which two different Tel2 pathways promote protein stabilization of Mec1 and Tel1 in budding yeast. PMID: 28827813
    2. In this study, we characterized functions of the FATC domain of Tel1 by introducing substitution or truncation mutations. PMID: 26246601
    3. once loaded to a double-strand break (DSB) by the MRX complex, promotes/stabilizes MRX association to the DSB in a positive feedback loop PMID: 26901759
    4. By performing a genetic screen, we identify rad53 and tel1 mutant alleles that suppress both the DNA damage hypersensitivity and the resection defect of sae2Delta cells through an Sgs1-Dna2-dependent mechanism PMID: 26584331
    5. In the absence of Tel1, a significant proportion of crossovers occur through a non-ZMM-dependent pathway, contributing to a crossover landscape with poor interference. PMID: 26305689
    6. Tel1 directs early replication and phosphorylates Rif1.Tel1 acts upstream of Rif1 in controlling telomere replication timing. PMID: 25329891
    7. Tel1 plays role in signal transduction during DNA replication. PMID: 25752575
    8. These results establish that Tel1(ATM) specifies not only the extent but also the timing of telomerase recruitment PMID: 25122631
    9. observations support a hierarchical view of recombination initiation where Tel1(ATM) prevents clusters of DNA double-strand breaks, and further suppresses DSBs within the surrounding chromosomal region PMID: 25539084
    10. Retaining a chrV L telomeric hph marker was significantly higher in tel1Delta, sae2Delta. PMID: 24699249
    11. Pch2 prevents Mec1/Tel1-mediated Hop1 phosphorylation occurring independently of Red1 in Saccharomyces cerevisiae meiosis. PMID: 24465650
    12. We show here that Tel1p and Rad53p, homologs of the mammalian DNA damage response kinases ATM and Chk2, mediate a hormetic mtROS longevity signal that extends yeast chronological lifespan. PMID: 23747251
    13. Tel1 is critical for DNA damage response and telomere homeostasis. PMID: 23378069
    14. Red1-SUMO-C interaction mediates both Mec1- and Tel1-mediated Hop1-T318 phosphorylation. PMID: 23775120
    15. The results show that Tel1 and Rad51 have multiple roles in the maintenance of vertebrate-like telomeres in yeast, supporting the idea that they may participate to evolutionary conserved telomere protection mechanisms acting at uncapped telomeres. PMID: 23677619
    16. Tel1 and Mec1, the budding yeast ATM and ATR, respectively, down-regulate Rec114 upon meiotic DSB formation through phosphorylation PMID: 23825959
    17. MRN/MRX recognizes double-strand break ends and recruits Tel1 to the DNA ends. Protein attachment at DNA ends enhances Tel1 activation through MRX function, whereas protein tethering in the middle of DNA fragments does not. PMID: 21402778
    18. Chromosome rearrangements and aneuploidy in yeast strains lacking both Tel1p and Mec1p reflect deficiencies in two different mechanisms PMID: 20534547
    19. Tel1 mutations are associated with increased rates of spontaneous loss of heterozygosity leading to genome instability. PMID: 20410660
    20. CDK1 complexes participate in triggering the Tel1-dependent checkpoint and are required to maintain both Mec1- and Tel1-dependent Rad53 phosphorylation, which are critical events in the DNA damage checkpoint cascade PMID: 15542824
    21. We present the characterization of a new mutation in the TEL1-gene, called tel1-11. Telomere and checkpoint functions are differentially affected by the mutant protein Tel1-11p. PMID: 16228207
    22. The viability of yku70Delta mec1Delta and yku80Delta mec1Delta cells depends on the ATM-related Tel1 kinase, the Mre11-Rad50-Xrs2 complex, and the DNA damage checkpoint protein Rad9 PMID: 16287875
    23. Sae2 phosphorylation depends on the checkpoint kinases Mec1 and Tel1, whose simultaneous deletion also impairs meiotic DNA double-strand break repair PMID: 16861895
    24. beta-lapachone activates a Mre11p-Tel1p checkpoint pathway in budding yeast PMID: 17106258
    25. These data argue that Mre11p and Tel1p affect telomere length by promoting telomerase recruitment to telomeres, whereas Mec1p has only a minor role in telomerase recruitment in a TEL1 cell. PMID: 17188035
    26. Tel1, the ATM orthologue, has two functions in checkpoint response to double-strand breaks. PMID: 17347674
    27. Sae2 and Tel1 promote microhomology-mediated end joining but inhibit non-homologous end joining, likely by regulating Mre11-dependent ssDNA accumulation at DNA break. PMID: 17565964
    28. These data suggest that Tel1p targets telomerase to the DNA ends most in need of extension. PMID: 17656141
    29. A chromatin remodeling complex is established as a functional component in the Mec1/Tel1 DNA damage signaling pathway that modulates checkpoint responses. PMID: 17693258
    30. Tel1p preferentially associates with the shortest telomeres and stimulates their elongation by telomerase. PMID: 17803948
    31. Telomerase repeat addition processivity is significantly increased at extremely short telomeres, a process that is dependent on the ATM-ortholog Tel1. PMID: 17908934
    32. Data show that Tel1 kinase and subtelomere-bound Tbf1 mediate preferential elongation of short telomeres by telomerase in yeast. PMID: 17917674
    33. Tel1-hy544 can activate the checkpoint more efficiently than wild-type Tel1 PMID: 17954565
    34. Study demonstrates that Mec1 and Tel1 promote meiotic interhomolog recombination by targeting the axial element protein Hop1. PMID: 18329363
    35. Data show that Tel1 and Tel2 interact, and that even when Tel1 protein levels are high, this interaction is specifically required for Tel1 localization to a DNA break and its activation of downstream targets. PMID: 18334620
    36. Mec1 and Tel1 inhibit anaphase in response to DNA damage by utilizing spindle assembly checkpoint proteins. PMID: 18454191
    37. Results define a conserved sequence motif within an otherwise poorly defined region of the Tel1/ATM kinase family proteins that is essential for normal Tel1 function in Saccharomyces cerevisiae. PMID: 18625723
    38. like human ATM, Tel1 serves as a key regulator for chromosome integrity in the pathway that reduces the risk for DSB-induced chromosome translocations, and are probably pertinent to the oncogenic chromosome translocations in ATM-deficient cells PMID: 18650924
    39. Budding yeasts carrying human-type telomeric repeats at their chromosome termini show a chronic activation of the Rad53-dependent DNA damage checkpoint pathway and a G2/M cell cycle delay. PMID: 19007917
    40. Tel1p has two separate functions in telomere maintenance and that the Xrs2p-dependent recruitment of Tel1p to telomeres plays an important role even in the absence of its kinase activity. PMID: 19596790

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  • 亚细胞定位：
    Nucleus. Chromosome, telomere. Note=Localizes to nuclear DNA repair foci in response to DNA double strand breaks.
  • 蛋白家族：
    PI3/PI4-kinase family, ATM subfamily
  • 数据库链接：

    KEGG: sce:YBL088C

    STRING: 4932.YBL088C

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