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  4. Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase GCN2 (GCN2), partial

Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase GCN2 (GCN2), partial

  • 中文名称：
    酿酒酵母GCN2重组蛋白
  • 货号：
    CSB-YP322210SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母GCN2重组蛋白
  • 货号：
    CSB-EP322210SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母GCN2重组蛋白
  • 货号：
    CSB-EP322210SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母GCN2重组蛋白
  • 货号：
    CSB-BP322210SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母GCN2重组蛋白
  • 货号：
    CSB-MP322210SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GCN2
  • Uniprot No.：
    P15442
  • 别名：
    GCN2; AAS1; YDR283C; eIF-2-alpha kinase GCN2; EC 2.7.11.1; General control non-derepressible protein 2; Serine/threonine-protein kinase GCN2
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/SUI2) on 'Ser-52' in response to low amino acid, carbon, or purine availability. Plays a role as an activator of the general amino acid control (GAAC) pathway required for adapatation to nutrient starvation. Converts phosphorylated eIF-2-alpha/SUI2 either to a competitive inhibitor of translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator GCN4, and hence allowing GCN4-mediated reprogramming of transcription to alleviate nutrient depletion. Binds uncharged tRNAs.; (orthologuous-specific) Binds to aminoacylated tRNA(Phe) less tightly than to deacylated tRNA(Phe). Binds to double-stranded RNA.
  • 基因功能参考文献：
    1. findings demonstrate that TORC1 is an effector of Gcn2 in amino acid signaling, hence defining a novel mechanism by which TORC1 senses amino acid starvation PMID: 28057755
    2. GCN2 is required for nitrogen catabolite repression-sensitive nuclear Gln3 localization. Gcn2 functions upstream of Ure2. PMID: 28007891
    3. Mutations leading to amino acid substitutions on this surface were identified that activate Gcn2 at low levels of tRNA binding (Gcd- phenotype), while other substitutions block kinase activation (Gcn- phenotype PMID: 25695491
    4. To mediate the activation of protein kinase Gcn2, Gcn1 interacts with Rps10. PMID: 25437641
    5. Gcd- substitutions enhance YKD-KD interactions in vitro, whereas Gcn- substitutions in alphaE and alphaI suppress both this effect and the constitutive activation of Gcn2 conferred by YKD Gcd- substitutions PMID: 24811037
    6. Data indicate that the crystal structures of murine and yeast GCN2 protein kinase C-terminal domain (CTD) showed dimers. PMID: 24719324
    7. Activation of Gcn2p by movement protein (MP) expression required a functional Tor1p kinase, since rapamycin treatment alleviated the yeast cell growth defect and blocked eIF2alpha phosphorylation triggered by MP expression PMID: 22087310
    8. the interaction between Pkh1 and Gcn2 was confirmed by co-immunoprecipitation and Pkh1 was shonwn to phosphorylate Gcn2 in vitro. PMID: 22326914
    9. purified eEF1A reduced the ability of Gcn2 to phosphorylate its substrate, eIF2alpha, but did not diminish Gcn2 autophosphorylation. PMID: 21849502
    10. Results highlight the importance of the GCN2 eIF2 kinase pathway for maintaining metabolic homeostasis, contributing to appropriate tRNA charging and growth adaptation in response to culture conditions deficient for tryptophan and arginine. PMID: 20684782
    11. structural analysis of GCN2 autoinhibition and mutational activation PMID: 15964839
    12. the salt bridge interaction and dimer interface observed in the PKR structure is critical for the activity of PKR, GCN2, and PERK PMID: 17202131
    13. Mutationally disrupting the hydrophobic network in various ways constitutively activates eIF2alpha phosphorylation in vivo and bypasses the requirement for a key tRNA binding motif (m2) and Thr-882 in Gcn2. PMID: 19114556
    14. analysis of tRNA charging and activation of the eIF2 kinase Gcn2p PMID: 19546227

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily
  • 数据库链接：

    KEGG: sce:YDR283C

    STRING: 4932.YDR283C

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