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  4. Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase CBK1 (CBK1), partial

Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase CBK1 (CBK1), partial

  • 中文名称：
    Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase CBK1(CBK1) ,partial
  • 货号：
    CSB-YP347240SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase CBK1(CBK1) ,partial
  • 货号：
    CSB-EP347240SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase CBK1(CBK1) ,partial
  • 货号：
    CSB-EP347240SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase CBK1(CBK1) ,partial
  • 货号：
    CSB-BP347240SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase CBK1(CBK1) ,partial
  • 货号：
    CSB-MP347240SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CBK1
  • Uniprot No.：
    P53894
  • 别名：
    CBK1; YNL161W; N1727Serine/threonine-protein kinase CBK1; EC 2.7.11.1; Cell wall biosynthesis kinase
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein kinase that seems to play a role in the regulation of cell morphogenesis and proliferation.
  • 基因功能参考文献：
    1. Cbk1 and Bck2 are required for Mpk1 activation during heat shock and cell wall stress and for Mpk1 dephosphorylation during heat shock adaptation. PMID: 22031291
    2. propose that Cdc14 coordinates cell separation with mitotic exit via FEAR-initiated phosphorylation of the Cbk1 HM site and MEN-activated reversal of mitotic CDK phosphorylations that block both Cbk1 and Ace2 function PMID: 21135117
    3. Cbk1 regulation of the RNA-binding protein Ssd1 integrates cell fate with translational control. PMID: 19962308
    4. Mutations in BRR1 or MPT5 lead to a restoration of fertility induced by cbk1p mutation, and a more-or-less pronounced restoration of polarity; they also show genetic interactions with SSD1. PMID: 19546315
    5. CBK1-S745F allele leads to a constitutively active form of Cbk1p that is independent of the upstream RAM network. PMID: 19967545
    6. Phosphoregulation of Cbk1 is critical for RAM network function. Intramolecular autophosphorylation of the enzyme's activation loop is critical for kinase activity but is only partially required for cell separation and polarized growth. PMID: 17145962
    7. Cbk1 exhibits a novel specificity that is likely conserved among related kinases from yeast to metazoans. Cbk1 is functionally restricted to the daughter cell, and cannot diffuse from the daughter to the mother. PMID: 18715118
    8. These data suggest that an important function for Cbk1 and the RAM signaling network is to regulate growth and secretion via Golgi and Sec2/Sec4-dependent processes. PMID: 18843045

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  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, COT1 subfamily
  • 数据库链接：

    KEGG: sce:YNL161W

    STRING: 4932.YNL161W

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