Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Saccharomyces cerevisiae Saccharopepsin (PEP4)

Recombinant Saccharomyces cerevisiae Saccharopepsin (PEP4)

In Stock
  • 中文名称：
    酿酒酵母PEP4重组蛋白
  • 货号：
    CSB-YP362092SVGa4
  • 规格：
    ￥2616
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    PEP4
  • Uniprot No.：
    P07267
  • 别名：
    (Aspartate protease)(Carboxypeptidase Y-deficient protein 4)(Proteinase YSCA)
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    48.8 kDa
  • 表达区域：
    77-405aa
  • 氨基酸序列
    GGHDVPLTNYLNAQYYTDITLGTPPQNFKVILDTGSSNLWVPSNECGSLACFLHSKYDHEASSSYKANGTEFAIQYGTGSLEGYISQDTLSIGDLTIPKQDFAEATSEPGLTFAFGKFDGILGLGYDTISVDKVVPPFYNAIQQDLLDEKRFAFYLGDTSKDTENGGEATFGGIDESKFKGDITWLPVRRKAYWEVKFEGIGLGDEYAELESHGAAIDTGTSLITLPSGLAEMINAEIGAKKGWTGQYTLDCNTRDNLPDLIFNFNGYNFTIGPYDYTLEVSGSCISAITPMDFPEPVGPLAIVGDAFLRKYYSIYDLGNNAVGLAKAI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-sumostar-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

相关产品

PEP4 Proteins

靶点详情

  • 功能：
    Aspartyl protease implicated in the post-translational regulation of S.cerevisiae vacuolar proteinases. Acts on YSCB, on YSCY and on itself.
  • 基因功能参考文献：
    1. Pep4 mutant cells are highly sensitive to oxidative and apoptotic stress induced by hydrogen peroxide and acetic acid. PMID: 29224051
    2. Study discuss the structure and physiological function of PEP4, and its transport routes: the Golgi-to-vacuole pathway and the constitutive Golgi-to-plasma membrane pathway. In addition, it is briefly discussed that calcium homeostasis plays an important role in the secretory pathway of proteins, implying that the regulation of PrA delivery to the plasma membrane requires the involvement of calcium ion. [review] PMID: 29124367
    3. The propeptide of yeast cathepsin D inhibits programmed necrosis. PMID: 21593793
    4. The finding that both mitochondrial AAC proteins and the vacuolar Pep4p interfere with mitochondrial degradation suggests a complex regulation and interplay between mitochondria and the vacuole in yeast programmed cell death.[Pep4 & AAC] PMID: 20345665
    5. concluded that increases in reactive-oxygen species and nuclear pore complex permeability occur relatively early during H2O2-induced cell death; later, Pep4p migrates out of vacuoles and degrades nucleoporins after the cells are effectively dead PMID: 16183335
    6. results indicate that Pep4p is important for protein turnover after oxidative damage; however, increased removal of oxidized proteins is not sufficient to enhance lifespan PMID: 17159212
    7. the lag phase of cell growth of partial PrA (PEP4) gene deletion mutant (36 h) and PrA-negative mutant (48 h) was significantly extended, compared with the wild type strain (24 h) (P<0.05), but PrA had no effect on glucose metabolism PMID: 19817002

    显示更多

    收起更多

  • 亚细胞定位：
    Vacuole. Note=Lysosome-like vacuoles.
  • 蛋白家族：
    Peptidase A1 family
  • 数据库链接：

    KEGG: sce:YPL154C

    STRING: 4932.YPL154C

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |