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  4. Recombinant Saccharomyces cerevisiae Regulatory protein GAL4 (GAL4), partial

Recombinant Saccharomyces cerevisiae Regulatory protein GAL4 (GAL4), partial

  • 中文名称：
    酿酒酵母GAL4重组蛋白
  • 货号：
    CSB-YP366145SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母GAL4重组蛋白
  • 货号：
    CSB-EP366145SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母GAL4重组蛋白
  • 货号：
    CSB-EP366145SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母GAL4重组蛋白
  • 货号：
    CSB-BP366145SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母GAL4重组蛋白
  • 货号：
    CSB-MP366145SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GAL4
  • Uniprot No.：
    P04386
  • 别名：
    GAL4; YPL248C; Regulatory protein GAL4
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
  • 基因功能参考文献：
    1. To facilitate application of the KZip(+) technology, we have developed a versatile set of LexAop-KZip(+) fly lines that can be used directly with the large number of LexA driver lines with known expression patterns. KZip(+) significantly sharpens the precision of neuronal genetic control available in Drosophila and may be extended to other organisms where Split GAL4-like systems are used. PMID: 28363977
    2. Report drosophila line in which the expression of yeast GAL4 is up-regulated in response to ingestion of silver nanoparticles PMID: 23827194
    3. Gal4 binds to GAL gene UASGAL (upstream activation sequence in GAL gene promoter) sites as a dimer via its N-terminal domain and activates transcription via a C-terminal transcription activation domain (AD). PMID: 23858060
    4. Double-transgenic mice in a GAL4/WNT1 system exhibit increased locomotor activity, decreased social interaction, and impaired short-term spatial memory and nesting behavior. PMID: 23134367
    5. Gal80 binds to the activation domain of Gal4 and prevents it from activating transcription. PMID: 22479149
    6. These data demonstrate that Gal80 is a powerful addition to the genetic techniques available to map and manipulate neural circuits in zebrafish. PMID: 21905164
    7. Data show that the DE-Gal4 line is a useful tool with which to manipulate gene expression in half of Drosophila developing eye. PMID: 19882738
    8. A chimeric transactivator in which the Gal4 DNA-binding domain is fused to the activation domain of viral protein VP16 (GAL4-VP16) tightly associates with yeast chromatin and reorganizes nucleosome arrays by an ATP-independent mechanism. PMID: 15766286
    9. Gal4 ubiquitylation and destruction are required for initiation-competent transcription complexes to transition to fully mature elongating complexes capable of appropriate mRNA processing. PMID: 15797387
    10. identified polypeptides in close physical proximity to Gal4 during transcription activation PMID: 16199885
    11. Review provides an updated account of Gal4 function, including data from new technologies that have been recently applied to the study of the GAL network. PMID: 16670683
    12. Under non-inducing conditions, the complex is dynamic, but on induction the Gal4-promoter complexes 'lock in' and exhibit long half-lives; inhibition of proteasome-mediated proteolysis had little or no effect on Gal4-mediated gene expression PMID: 16929306
    13. The chimeric transcription factor Gal4-VP16 can enhance SWI/SNF histone octamer transfer activity, resulting in targeted histone eviction from a nucleosome probe. PMID: 17235287
    14. Increased gal4 dosage correlated with accumulation of insoluble GAL4, suggesting that the cascade of events leading to apoptosis might be triggered by protein deposits of either GAL4 or protein intermediates PMID: 17313569
    15. An engineered yeast Gal4 transcription activator construct illustrates a method that is applicable to functional studies of neural circuits in transgenic zebrafish. PMID: 18202183
    16. This study validates the physiological importance of Gal4 monoubiquitylation and clarifies its major role as that of protecting the activator from being destabilized by the proteasomal ATPases. PMID: 18326036
    17. A report on the structure of a complete Gal4 dimer bound to DNA and additional biochemical studies to address the molecular basis for Gal4 dimerization in DNA binding. PMID: 18611375
    18. In the presence of galactose, Gal4p-YFP remained in the nucleus. PMID: 18952899
    19. These results, using the Gal4BD-Spo11 fusion protein, demonstrate that Spo11 itself has sequence preference and contributes to the choice of DNA double-strand break positions. PMID: 19380488
    20. Gal4p can be crosslinked to Gal80p with bis(sulphosuccinimidyl) suberate. PMID: 11964151

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: sce:YPL248C

    STRING: 4932.YPL248C

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