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  4. Recombinant Saccharomyces cerevisiae Protein transport protein DSL1 (DSL1), partial

Recombinant Saccharomyces cerevisiae Protein transport protein DSL1 (DSL1), partial

  • 中文名称：
    酿酒酵母DSL1重组蛋白
  • 货号：
    CSB-YP346892SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母DSL1重组蛋白
  • 货号：
    CSB-EP346892SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母DSL1重组蛋白
  • 货号：
    CSB-EP346892SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母DSL1重组蛋白
  • 货号：
    CSB-BP346892SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母DSL1重组蛋白
  • 货号：
    CSB-MP346892SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DSL1
  • Uniprot No.：
    P53847
  • 别名：
    DSL1; YNL258C; N0842Protein transport protein DSL1; Dependent on SLY1-20 protein 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for protein transport between the Golgi and the endoplasmic reticulum. May tether coatomer-coated retrograde transport vesicles to the ER membrane through interaction with coatomer as well as the SNARE complex. May contribute to the stabilization of the SNARE complex.
  • 基因功能参考文献：
    1. SNARE-mediated homotypic ER fusion requires DSL1. PMID: 25187651
    2. subunits of the Dsl1 complex (Dsl1, Dsl3, and Tip20), as well as the SNAREs Ufe1 and Sec20, are ER-resident proteins that do not seem to move into COPII vesicles. PMID: 21550981
    3. a direct role of the Dsl1 complex, in particular Tip20p, in the formation and stabilization of ER SNARE complexes. PMID: 21482823
    4. Studies indicate that Dsl1 tethering complex seems to be designed to expose an unstructured domain of Dsl1p at its top, which is required to capture vesicles. PMID: 20226673
    5. DSL1 is required for the stable interaction of the SNARE Use1p with a central subcomplex consisting of Tip20p and the SNARE proteins Ufe1p and Sec20p. PMID: 15958492
    6. SEC20, SEC39, and DSL1 are essential secretory genes involved in the early stages of peroxisome assembly PMID: 19346454
    7. Study determined the structure of the Dsl1 complex, revealing a tower containing at its base the binding sites for two endoplasmic reticulum SNAREs and at its tip a flexible lasso for capturing vesicles. PMID: 20005805

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein.
  • 数据库链接：

    KEGG: sce:YNL258C

    STRING: 4932.YNL258C

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