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  4. Recombinant Saccharomyces cerevisiae Protein DOA1 (DOA1), partial

Recombinant Saccharomyces cerevisiae Protein DOA1 (DOA1), partial

  • 中文名称：
    酿酒酵母DOA1重组蛋白
  • 货号：
    CSB-YP327478SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母DOA1重组蛋白
  • 货号：
    CSB-EP327478SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母DOA1重组蛋白
  • 货号：
    CSB-EP327478SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母DOA1重组蛋白
  • 货号：
    CSB-BP327478SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母DOA1重组蛋白
  • 货号：
    CSB-MP327478SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DOA1
  • Uniprot No.：
    P36037
  • 别名：
    DOA1; UFD3; ZZZ4; YKL213CProtein DOA1; Degradation of alpha protein 1; Ubiquitin fusion degradation protein 3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ubiquitin-binding protein involved in protein ubiquitination, sorting and degradation. Acts as a ubiquitinated substrate-recruiting adapter for chaperone ATPase CDC48 by binding mono- or polyubiquitin chains. Depending on the context, promotes or prevents proteasomal degradation of ubiquitinated proteins. Involved in the ubiquitin fusion degradation (UFD) pathway by promoting the degradation of ubiquitinated proteins. Involved in the mitochondria-associated degradation pathway (MAD) by promoting the degradation of several ubiquitinated membrane proteins. By competing with UFD2 to bind CDC48, prevents the multi-ubiquitination and subsequent degradation of UFD2-dependent substrates. Required for ribophagy, a process which relocalizes ribosomal particles into the vacuole for degradation in response to starvation. Involved in the ubiquitin-mediated sorting of membrane proteins into multivesicular bodies (MVBs). In addition, plays an essential role in maintaining cellular ubiquitin levels. May affect indirectly the degradation of ubiquitinylated proteins by regulating cellular ubiquitin levels.
  • 基因功能参考文献：
    1. Small-angle X-ray scattering combined with molecular docking and biochemical analysis yield the solution structure of the Doa1/PFU:Hse1/SH3 complex and suggest that hydrogen bonding is a major determinant in the interaction of Doa1/PFU with Hse1/SH3. PMID: 24607902
    2. A structural comparison of Doa1 with Ufd2 revealed that they share a similar ARM-like repeat, supporting a model in which Doa1 and Ufd2 compete for Cdc48 binding and may dictate the fate of ubiquitinated proteins in the proteasome pathway PMID: 21063153
    3. The data suggest that Doa1 is the major source of ubiquitin for the DNA damage response and that Doa1 also plays an additional essential and more specific role in the monoubiquitination of histone H2B. PMID: 16705165
    4. DOA1/UFD3 plays a role in sorting ubiquitinated membrane proteins into multivesicular bodies PMID: 18508771
  • 亚细胞定位：
    Nucleus. Cytoplasm. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    WD repeat PLAP family
  • 数据库链接：

    KEGG: sce:YKL213C

    STRING: 4932.YKL213C

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