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  4. Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 (PRP28), partial

Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 (PRP28), partial

  • 中文名称：
    酿酒酵母PRP28重组蛋白
  • 货号：
    CSB-YP340539SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母PRP28重组蛋白
  • 货号：
    CSB-EP340539SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母PRP28重组蛋白
  • 货号：
    CSB-EP340539SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母PRP28重组蛋白
  • 货号：
    CSB-BP340539SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母PRP28重组蛋白
  • 货号：
    CSB-MP340539SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PRP28
  • Uniprot No.：
    P23394
  • 别名：
    PRP28; YDR243C; YD8419.10CPre-mRNA-splicing ATP-dependent RNA helicase PRP28; EC 3.6.4.13; Helicase CA8
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATP-dependent RNA helicase involved in mRNA splicing. May destabilize the U1/5'-splice site duplex to permit an effective competition for the 5'-splice site by the U6 snRNA, resulting in the switch between U1 and U6 at the 5'-splice site. May also act to unwind the U4/U6 base-pairing interaction in the U4/U6/U5 snRNP, facilitating the first covalent step of splicing.
  • 基因功能参考文献：
    1. The study demonstrated that yeast Prp28 is a bona fide RNA-dependent ATPase. PMID: 25303995
    2. The Prp8 suppressor partially corrected both the early assembly and later activation defects of prp28-1, supporting a role for this U5 snRNP protein in both the ATP-independent and ATP-dependent functions of Prp28. PMID: 24231520
    3. The subdomain linker in Prp28p plays important roles both in splicing efficiency and in proofreading of 5' splice site. PMID: 23462954
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    DEAD box helicase family, DDX23/PRP28 subfamily
  • 数据库链接：

    KEGG: sce:YDR243C

    STRING: 4932.YDR243C

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