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  4. Recombinant Saccharomyces cerevisiae Pleiotropic ABC efflux transporter of multiple drugs (PDR5), partial

Recombinant Saccharomyces cerevisiae Pleiotropic ABC efflux transporter of multiple drugs (PDR5), partial

  • 中文名称：
    酿酒酵母PDR5重组蛋白
  • 货号：
    CSB-YP330240SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母PDR5重组蛋白
  • 货号：
    CSB-EP330240SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母PDR5重组蛋白
  • 货号：
    CSB-EP330240SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母PDR5重组蛋白
  • 货号：
    CSB-BP330240SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母PDR5重组蛋白
  • 货号：
    CSB-MP330240SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PDR5
  • Uniprot No.：
    P33302
  • 别名：
    PDR5; LEM1; STS1; YDR1; YOR153W; Pleiotropic ABC efflux transporter of multiple drugs; Pleiotropic drug resistance protein 5; Suppressor of toxicity of sporidesmin
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Active efflux of weakly charged organic compounds of 90 cubic Angstroms to 300 cubic Angstroms surface volume. Confers resistance to numerous chemicals including cycloheximide, sulfomethuron methyl, steroids, antiseptics, antibiotics, anticancer, herbicides, mycotoxins, insecticides, ionophores, alkaloids, flavonoids, phenothiazines, organotin compounds, carbazoles, lysosomotropic aminoesters, detergents, rhodamines and other fluorophores, azoles and other antifungals. Exhibits nucleoside triphosphatase activity.
  • 基因功能参考文献：
    1. These results present new evidence, suggesting that transmembrane helix 2 plays an important role for the efflux function of Pdr5p. PMID: 27189366
    2. structural and functional implications for Pdr5 PMID: 27543784
    3. High-Copy Overexpression Screening Reveals PDR5 as the Main Doxorubicin Resistance Gene in Yeast. PMID: 26690737
    4. Pdr5 analyses are applicable to mammalian asymmetric ABC multidrug transporters, including transporter promiscuity, function of non-catalytic ATP-binding site, description of a transmission interface, and discovery that Pdr5 is a molecular diode. Review. PMID: 25886173
    5. Novel phenotype of an S1368A mutant that lies in the putative drug-binding pocket of the yeast multidrug transporter Pdr5. PMID: 25112867
    6. We describe here the first attempts to restore complete symmetry in an asymmetric ABC transporter and to study its effects, which might be relevant to the entire class of asymmetric ABC transporters. PMID: 24733388
    7. Mutations adjacent to the end of transmembrane helices 6 and 7 independently affect drug efflux capacity of Pdr5p. PMID: 24333836
    8. The deviant ATP-binding site of the multidrug efflux pump Pdr5 plays an active role in the transport cycle. PMID: 24019526
    9. These results indicate that the S1360F mutation within the transmembrane domain interferes drastically with the ability of the nucleotide-binding domains to hydrolyze ATP by interfering with interdomain crosstalk. PMID: 23464591
    10. A mutation in intracellular loop 4 affects the drug-efflux activity of the yeast multidrug resistance ABC transporter Pdr5p. PMID: 22238618
    11. activity of Pdr5 in a strain with wild-type PDR1 allele was shown to drop sharply on glucose depletion from the medium and then again at the end of the diauxic shift when the cells are adapted to growth on respiratory substrates. PMID: 15471577
    12. Thus, it was found that enniatins are potent and specific inhibitors for Pdr5p, with less toxicities than that of FK506. PMID: 15707993
    13. membrane Pdr5p levels diminished rapidly during incubation with high calcium in a manner dependent on calcineurin PMID: 15849433
    14. These results suggest that Pdr5 requires a properly folded nucleotide-binding domain for trafficking to the plasma membrane. PMID: 17302805
    15. Using purified plasma membrane vesicles, we demonstrate that Pdr5p can use GTP to fuel substrate transport. We propose that Pdr5p increases its multidrug transport substrate specificity by using more than one nucleotide as an energy source. PMID: 17956128
    16. provide important insights into the molecular mechanism of Pdr5 and suggest that not solely the transmembrane domains dictate substrate selection PMID: 18356296
    17. drug sensitivity of mutant Pdr5 is attributable to the uncoupling of NTPase activity and transport PMID: 18842589

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Note=The ERAD mutants 'Pro-183' and 'Tyr-1427' fail to reach the plasma membrane. The mutant 'Pro-183' accumulates into ER-associated compartments.
  • 蛋白家族：
    ABC transporter superfamily, ABCG family, PDR (TC 3.A.1.205) subfamily
  • 数据库链接：

    KEGG: sce:YOR153W

    STRING: 4932.YOR153W

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