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  4. Recombinant Saccharomyces cerevisiae Phospholipid:diacylglycerol acyltransferase (LRO1), partial

Recombinant Saccharomyces cerevisiae Phospholipid:diacylglycerol acyltransferase (LRO1), partial

  • 中文名称：
    酿酒酵母LRO1重组蛋白
  • 货号：
    CSB-YP328100SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母LRO1重组蛋白
  • 货号：
    CSB-EP328100SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母LRO1重组蛋白
  • 货号：
    CSB-EP328100SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母LRO1重组蛋白
  • 货号：
    CSB-BP328100SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母LRO1重组蛋白
  • 货号：
    CSB-MP328100SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    LRO1
  • Uniprot No.：
    P40345
  • 别名：
    LRO1; YNR008W; N2042; Phospholipid:diacylglycerol acyltransferase; PDAT; EC 2.3.1.158
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes triacylglycerol (TAG) formation by an acyl-CoA independent pathway. The enzyme specifically transfers acyl groups from the sn-2 position of a phospholipid to diacylglycerol (DAG), thus forming an sn-1-lysophospholipid. The preferred acyl donors are phosphatidylethanolamine (PE) and phosphatidylcholine (PC). Also capable of using broad acyl donors such as phosphatidic acid (PA), phosphatidylserine (PS), phosphatidylglycerol (PG) and phosphatidylinositol (PI), as well as monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), and acyl-CoA, and it is more likely to use unsaturated acyl donors. As acyl acceptors, it prefers 1,2- over 1,3-diacylglycerol (DAG). Additionally, has esterification activity that can utilize methanol as acyl acceptor to generate fatty acid methyl esters (FAME). Can also utilize ceramide instead of DAG, acylating the ceramides by attaching a fatty acid to the hydroxy group on the first carbon atom of the long-chain base to produce 1-O-acylceramides. Involved in lipid particle synthesis from the endoplasmic reticulum, promoting localized TAG production at discrete ER subdomains. Relocates from the endoplasmic reticulum to a subdomain of the inner nuclear membrane upon nutrient starvation, where it provides a site of TAG synthesis, which is coupled with nuclear membrane remodeling.
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass type II membrane protein. Nucleus inner membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 数据库链接：

    KEGG: sce:YNR008W

    STRING: 4932.YNR008W

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