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  4. Recombinant Saccharomyces cerevisiae Oligopeptide transporter 1 (OPT1), partial

Recombinant Saccharomyces cerevisiae Oligopeptide transporter 1 (OPT1), partial

  • 中文名称：
    酿酒酵母OPT1重组蛋白
  • 货号：
    CSB-YP334785SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母OPT1重组蛋白
  • 货号：
    CSB-EP334785SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母OPT1重组蛋白
  • 货号：
    CSB-EP334785SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母OPT1重组蛋白
  • 货号：
    CSB-BP334785SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母OPT1重组蛋白
  • 货号：
    CSB-MP334785SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    OPT1
  • Uniprot No.：
    P40897
  • 别名：
    OPT1; GSH11; HGT1; YJL212C; HRD799; J0236; Oligopeptide transporter 1; High affinity glutathione transporter 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    High affinity transporter for glutathione. Also transports tetra- and pentapeptides like the opioids leucine enkephalin (Tyr-Gly-Gly-Phe-Leu) and methionine enkephalin (Tyr-Gly-Gly_Phe-Met) across the cell membrane.
  • 基因功能参考文献：
    1. Data suggest that protonated residues E/D135 or N710 in Hgt1p prevent substrate/glutathione from binding at high pH; mutation of these residues allows substrate/glutathione to bind in a proton-dependent manner through involvement of other residues. PMID: 28389436
    2. ScOPT1 and AtOPT4 are proton-coupled OPTs with broad but distinct substrate specificities and affinities. PMID: 16149917
    3. The cysteine-free Hgt1p was nonfunctional and pointed to a critical role being played by the native cysteine residues of Hgt1p. PMID: 19496824
    4. Asn-124 in transmembrane domain 1(TMD1), Gln-222 in TMD4, Gln-526 in TMD9, and Glu-544, Arg-554, and Lys-562 in the intracellular loop are essential for the transport activity of Hgt1. PMID: 19589778
  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Oligopeptide OPT transporter family
  • 数据库链接：

    KEGG: sce:YJL212C

    STRING: 4932.YJL212C

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