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  4. Recombinant Saccharomyces cerevisiae Nuclear polyadenylated RNA-binding protein 3 (NAB3), partial

Recombinant Saccharomyces cerevisiae Nuclear polyadenylated RNA-binding protein 3 (NAB3), partial

  • 中文名称：
    酿酒酵母NAB3重组蛋白
  • 货号：
    CSB-YP340041SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母NAB3重组蛋白
  • 货号：
    CSB-EP340041SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母NAB3重组蛋白
  • 货号：
    CSB-EP340041SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母NAB3重组蛋白
  • 货号：
    CSB-BP340041SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母NAB3重组蛋白
  • 货号：
    CSB-MP340041SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NAB3
  • Uniprot No.：
    P38996
  • 别名：
    NAB3; HMD1; YPL190C; Nuclear polyadenylated RNA-binding protein 3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be required for packaging pre-mRNAs into ribonucleoprotein structures amenable to efficient nuclear RNA processing. Binds to poly(A)+ RNA. Appears to act in the maintenance of CLN3 mRNA levels.
  • 基因功能参考文献：
    1. Kinetic cross-linking and analysis of cDNAs uncovers a role for Nab3 in determining gene expression profiles during stress. PMID: 28400552
    2. Study analyzed the functional role of the prion-like low complexity domains (LCDs) of Nab3, which can assemble into a robust amyloid form. Results show that Nab3 LCD can be exchanged for the low complexity regions of other proteins as the prion protein Sup35, or the RNA-binding protein, Hrp1. Also, its C-terminus seems more complex than previously appreciated. PMID: 29023495
    3. a role for Nab3 in regulating the cellular response to nutrient availability PMID: 22431520
    4. Nab3 protein contains a self-assembly domain found in human heterogeneous nuclear ribonucleoprotein-C (hnRNP-C) that is necessary for transcription termination PMID: 23192344
    5. Structural homology modeling suggests that the carboxy-terminal amino acids of Nab3 forms an alpha-helical multimerization domain that helps assemble it onto an RNA substrate. PMID: 22564898
    6. the solution structure of the RNA-recognition motif (RRM) of Nab3 in complex with a UCUU oligonucleotide, representing the Nab3 termination element PMID: 21084293
    7. The crystal structure of the Nab3 RNA recognition motif reveals clear density for a UCU trinucleotide and a fourth putative U binding site. PMID: 20805243

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  • 亚细胞定位：
    Nucleus, nucleoplasm.
  • 数据库链接：

    KEGG: sce:YPL190C

    STRING: 4932.YPL190C

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