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  4. Recombinant Saccharomyces cerevisiae Nitrogen regulatory protein GLN3 (GLN3), partial

Recombinant Saccharomyces cerevisiae Nitrogen regulatory protein GLN3 (GLN3), partial

  • 中文名称：
    酿酒酵母GLN3重组蛋白
  • 货号：
    CSB-YP325989SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母GLN3重组蛋白
  • 货号：
    CSB-EP325989SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母GLN3重组蛋白
  • 货号：
    CSB-EP325989SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母GLN3重组蛋白
  • 货号：
    CSB-BP325989SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母GLN3重组蛋白
  • 货号：
    CSB-MP325989SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GLN3
  • Uniprot No.：
    P18494
  • 别名：
    GLN3; YER040WNitrogen regulatory protein GLN3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Positive nitrogen regulatory protein. Required for the activation of transcription of a number of genes (including the allantoin pathway genes) in response to the replacement of glutamine by glutamate as source of nitrogen. Binds the nitrogen upstream activation sequence of GLN1, the gene encoding glutamine synthetase. URE2 may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.
  • 基因功能参考文献：
    1. two different ways in which intracellular Gln3 localization is regulated PMID: 26333687
    2. nuclear localization domains of GATA activator Gln3 are required for transcription of target genes, presumably through dephosphorylation PMID: 25641578
    3. Ppz1 controls Gln3 localization by regulating its phosphorylation state in combination with Sat4 and Hal5. PMID: 21237705
    4. Gln3 localization in Saccharomyces cerevisiae is separable from and can overwhelm nitrogen source regulation PMID: 17439949
    5. there is a role for Golgi-to-endosome vesicular trafficking in TORC1-controlled nuclear translocation of Gln3 PMID: 18443284

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: sce:YER040W

    STRING: 4932.YER040W

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