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  4. Recombinant Saccharomyces cerevisiae Mitotic check point protein BFA1 (BFA1), partial

Recombinant Saccharomyces cerevisiae Mitotic check point protein BFA1 (BFA1), partial

  • 中文名称：
    Recombinant Saccharomyces cerevisiae Mitotic check point protein BFA1(BFA1) ,partial
  • 货号：
    CSB-YP344036SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Mitotic check point protein BFA1(BFA1) ,partial
  • 货号：
    CSB-EP344036SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Mitotic check point protein BFA1(BFA1) ,partial
  • 货号：
    CSB-EP344036SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Mitotic check point protein BFA1(BFA1) ,partial
  • 货号：
    CSB-BP344036SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae Mitotic check point protein BFA1(BFA1) ,partial
  • 货号：
    CSB-MP344036SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    BFA1
  • Uniprot No.：
    P47113
  • 别名：
    BFA1; YJR053W; J1667Mitotic check point protein BFA1; Cell cycle arrest protein BFA1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of a checkpoint which monitors spindle integrity and prevents premature exit from mitosis. This cell-cycle arrest depends upon inhibition of the G-protein TEM1 by the BFA1/BUB2 complex.
  • 基因功能参考文献：
    1. Tem1 activity is tightly controlled in mitosis by Bub2 and Bfa1 and is also regulated by the spindle orientation checkpoint that monitors the correct alignment of the mitotic spindle with the mother-daughter axis. Here is described the purification of Tem1, Bfa1, and Bub2 and a detailed radioactive filter-binding assay to study the nucleotide binding properties of Tem1 and the role of its regulators Bfa1 and Bub2. PMID: 27826857
    2. Bmh1 is a spindle position checkpoint (SPOC) component that breaks Bfa1 centrosome asymmetry upon SPOC activation. PMID: 24850890
    3. Cdc5 induces the asymmetric distribution of Bfa1 to the bud-directed spindle pole body independently of Bfa1 GAP activity at anaphase. PMID: 22253605
    4. various checkpoint signals are transmitted to the C-terminal domain of Bfa1 (Bfa1-D8(391-574)) and Bfa1p localization to spindle pole body is necessary but not sufficient to regulate mitotic exit in response to various checkpoint signals PMID: 15147270
    5. Bfa1 disappearance from the mother bound spindle pole body at the onset of anaphase, along with Cdc5 function, helps prompt Tem1 activation in telophase. PMID: 16449187
    6. Results suggest that the GTPase-activating activity of Bfa1p-Bub2p is responsible for the mitotic arrest caused by spindle damage and Bfa1p overproduction. PMID: 16682821
    7. there is a GAP-independent surveillance mechanism of Bfa1/Bub2, which, together with the GTP/GDP switch of Tem1, may be required for the genomic stability of cells with misaligned spindles PMID: 18667533
    8. A link is confirmed between the subcellular localization pattern of Bfa1 at the spindle pole bodies with cell cycle progression. PMID: 19948498

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, spindle pole.
  • 数据库链接：

    KEGG: sce:YJR053W

    STRING: 4932.YJR053W

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