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  4. Recombinant Saccharomyces cerevisiae Mitochondrial FAD-linked sulfhydryl oxidase ERV1 (ERV1)

Recombinant Saccharomyces cerevisiae Mitochondrial FAD-linked sulfhydryl oxidase ERV1 (ERV1)

  • 中文名称：
    酿酒酵母ERV1重组蛋白
  • 货号：
    CSB-YP340921SVG
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    ERV1
  • Uniprot No.：
    P27882
  • 别名：
    (14 kDa regulatory protein)(Essential for respiration and vegetative growth protein 1)
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    22.5 kDa
  • 表达区域：
    1-189aa
  • 氨基酸序列
    MKAIDKMTDNPPQEGLSGRKIIYDEDGKPCRSCNTLLDFQYVTGKISNGLKNLSSNGKLAGTGALTGEASELMPGSRTYRKVDPPDVEQLGRSSWTLLHSVAASYPAQPTDQQKGEMKQFLNIFSHIYPCNWCAKDFEKYIRENAPQVESREELGRWMCEAHNKVNKKLRKPKFDCNFWEKRWKDGWDE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    C-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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ERV1 Proteins

靶点详情

  • 功能：
    FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. Required for the import and folding of small cysteine-containing proteins in the mitochondrial intermembrane space (IMS). Forms a redox cycle with MIA40 that involves a disulfide relay system. Important for maintaining the cysteine residues in MIA40 in an oxidized state. Reduced ERV1 is reoxidized by cytochrome c. Required for the maturation of cytoplasmic, but not of mitochondrial Fe/S proteins.
  • 基因功能参考文献：
    1. Chimeras between the N-terminal arm of ScErv1 and a variety of truncated LtErv constructs were able to rescue yeast cells that lack ScErv1. PMID: 29310075
    2. Authors show that the fumarate reductase Osm1, which facilitates electron transfer from fumarate to succinate, fills this gap as a new electron acceptor. In addition to microsomes, Osm1 localizes to the mitochondrial intermembrane space and assembles with Erv1 in a complex. PMID: 28814504
    3. Role of tryptophan residues of Erv1: Trp95 and Trp183 are important for its folding and oxidase function. PMID: 26221027
    4. these results confirm that GSH is critical for cytosolic Fe-S protein biogenesis and iron regulation, whereas ruling out significant roles for Erv1 or Mia40 in these pathways. PMID: 26396185
    5. Mia40 serves as an electron sink in the Mia40-Erv1 protein import pathway. PMID: 26085103
    6. Taken together, we conclude that both shuttle cysteine residues are required for Erv1 function, and play complementary, but distinct, roles to ensure rapid turnover of active Erv1. PMID: 24625320
    7. Mia40 in cooperation with Erv1 promotes the formation of two disulfide bonds in the substrate protein, ensuring the efficiency of oxidative folding in the intermembrane space of mitochondria. PMID: 22918950
    8. These findings provide structural insights into electron transfer from Mia40 via the shuttle domain of one subunit of Erv1 to the CTD of another Erv1 subunit. PMID: 22910915
    9. established that the N-terminal shuttle domain of Erv1 is necessary and sufficient for interaction with Mia40. PMID: 20367271
    10. demonstrate that Erv1 dimerizes noncovalently and that the subunits of this homodimer cooperate in intersubunit electron exchange. PMID: 20188670
    11. Sudies demonstrate that Mia40, Erv1, and oxygen are the minimal machinery for Tim13 oxidation. PMID: 19477928
    12. We propose that Erv1 and Mia40 function as a disulfide relay system that catalyzes the import of proteins into the IMS by an oxidative folding mechanism. PMID: 15989955
    13. Mia40 and Erv1, cooperate in the assembly pathway of small proteins of the mitochondrial intermembrane space PMID: 16181637
    14. analysis of a disulfide relay system consisting of Mia40C and Erv1p PMID: 17959605
    15. Erv1 utilizes diverse pathways for electron shuttling in the mitochondrial intermembrane space, including using cytochrome c and cytochrome c peroxidase PMID: 17972915
    16. Oxidation driven by Mia40 and Erv1 determines vectorial transport of the precursors into the mitochondrial intermembrane space. PMID: 17978092
    17. Erv1 promotes disulfide transfer to the precursor while both oxidase and precursor are associated with the disulfide carrier Mia40. PMID: 18852299
    18. zinc is a powerful inhibitor of Erv1, an essential component of the import pathway used by the small Tim9,10 proteins PMID: 19117943
    19. there is both intersubunit and intermolecular electron transfer at shuttle and active site disulfides s of mitochondrial sulfhydryl oxidase Erv1p PMID: 19679655

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  • 亚细胞定位：
    Mitochondrion intermembrane space.
  • 数据库链接：

    KEGG: sce:YGR029W

    STRING: 4932.YGR029W

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