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  4. Recombinant Saccharomyces cerevisiae Minichromosome maintenance protein 10 (MCM10), partial

Recombinant Saccharomyces cerevisiae Minichromosome maintenance protein 10 (MCM10), partial

  • 中文名称：
    酿酒酵母MCM10重组蛋白
  • 货号：
    CSB-YP327128SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母MCM10重组蛋白
  • 货号：
    CSB-EP327128SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母MCM10重组蛋白
  • 货号：
    CSB-EP327128SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母MCM10重组蛋白
  • 货号：
    CSB-BP327128SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母MCM10重组蛋白
  • 货号：
    CSB-MP327128SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MCM10
  • Uniprot No.：
    P32354
  • 别名：
    MCM10; DNA43; YIL150CMinichromosome maintenance protein 10; Protein DNA43
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for DNA synthesis. Required for entry into or completion of S phase. Involved in DNA replication and seems to participate in the activation of the pre-replication complex (pre-RC) and in transcription elongation. May play a role as a key coordinator in assembling the replication fork. Proposed to function at replication origins following the binding of the MCM2-7 complex prior to the recruitment of CDC45. Probably is required to stimulate phosphorylation of the MCM2-7 complex by the CDC7-DBF4 kinase complex. May recruit the DNA polymerase alpha:primase complex to replication origins and is required to maintain it on chromatin independently of CDC45. May also play a role in transcriptional silencing.
  • 基因功能参考文献：
    1. This report demonstrates that Mcm10, an essential replication protein unique to eukaryotes, binds the replicative helicase CMG and greatly stimulates its helicase activity in vitro. Most significantly, Mcm10 enables CMG and the replisome to bypass blocks on the non-tracking DNA strand. PMID: 28869037
    2. These data suggest that DNA binding by Mcm10 is essential for the initiation of DNA replication. PMID: 27751725
    3. Mcm10 deficiency causes defective-replisome-induced mutagenesis and a dependency on error-free postreplicative repair. PMID: 24674891
    4. In the absence of Mcm10, a stable Cdc45-Mcm-GINS (CMG) complex was assembled at origins. However subsequent translocation of CMG, replication protein A loading to origins, and the intra-S checkpoint activation were severely diminished. PMID: 22285032
    5. These data indicate that Ctf4p facilitates Mcm10p to promote the DNA replication. PMID: 20381454
    6. Mcm10 plays two important roles as a linker of the MCM helicase and Polalpha at the elongating replication fork--first, to coordinate the activities of these two molecular motors PMID: 19917723
    7. required for the presence of DNA pol I at replication forks and may coordinate DNA synthesis with DNA unwinding PMID: 15494305
    8. Mcm10 is required for the maintenance of transcriptional silencing in Saccharomyces cerevisiae. PMID: 16085704
    9. Mcm10 mutants disrupt mating loci silencing and Mcm10 interacts with Sir2 and Sir3, suggesting that Mcm10 plays an essential, and separable role in transcriptional silencing. PMID: 16328881
    10. Mcm10 is required for the coupling of the replication and silencing machineries to silence chromatin in a context outside of DNA replication beyond the recruitment and spreading of Sir2 on chromatin. PMID: 19064704
    11. analysis of DNA binding properties of the Mcm10 protein from Saccharomyces cerevisiae PMID: 19605346

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  • 亚细胞定位：
    Nucleus. Note=Colocalizes with ORC1 on chromatin independent from cell cycle. According to PubMed:15494305 is recruited to replication origins in a cell cycle regulated manner.
  • 蛋白家族：
    MCM10 family
  • 数据库链接：

    KEGG: sce:YIL150C

    STRING: 4932.YIL150C

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