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  4. Recombinant Saccharomyces cerevisiae Kinetochore protein NDC80 (TID3), partial

Recombinant Saccharomyces cerevisiae Kinetochore protein NDC80 (TID3), partial

  • 中文名称：
    酿酒酵母NDC80重组蛋白
  • 货号：
    CSB-YP331173SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母NDC80重组蛋白
  • 货号：
    CSB-EP331173SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母NDC80重组蛋白
  • 货号：
    CSB-EP331173SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母NDC80重组蛋白
  • 货号：
    CSB-BP331173SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母NDC80重组蛋白
  • 货号：
    CSB-MP331173SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NDC80
  • Uniprot No.：
    P40460
  • 别名：
    NDC80; HEC1; TID3; YIL144W; Kinetochore protein NDC80; 80 kDa spindle component protein; Nuclear division cycle protein 80; Two-hybrid interaction with DMC1 protein 3
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.
  • 基因功能参考文献：
    1. Thus, expression of a 5' extended mRNA isoform causes transcriptional interference at the downstream promoter of NDC80. The authors demonstrate that this is an effective mechanism to promote dynamic changes in gene expression during cell differentiation. PMID: 28906248
    2. The authors discovered that in budding yeast, kinetochore inactivation during meiosis occurs by reducing the abundance of a limiting subunit, Ndc80. PMID: 28906249
    3. Ndc80 helical hairpin, microtubule-binding and the tetramerization domains are essential for Ndc80 function. PMID: 23833183
    4. We found that kinetochore-bound Ndc80 is phosphorylated on Ipl1 sites in vivo, but this phosphorylation is not essential.Instead, we show that additional Ipl1 targets contribute to segregation and the spindle checkpoint. PMID: 19822728
    5. data on the four-protein Ndc80 complex suggests that the Spc24p/Spc25p end of the rod faces the centromere and the Ndc80p/Nuf2p end faces a spindle microtubule PMID: 15809444
    6. Results suggest that the conformational kink in Ndc80 is essential for correct kinetochore geometry and could be part of a tension-sensing mechanism at the kinetochore. PMID: 18793650
    7. Findings demonstrate how an ensemble of Ndc80 complexes may provide the combination of plasticity and strength that allows kinetochores to maintain load-bearing tip attachments during both microtubule assembly and disassembly. PMID: 19269365

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  • 亚细胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Note=Associated with kinetochores.
  • 蛋白家族：
    NDC80/HEC1 family
  • 数据库链接：

    KEGG: sce:YIL144W

    STRING: 4932.YIL144W

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