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  4. Recombinant Saccharomyces cerevisiae Karyogamy protein KAR9 (KAR9), partial

Recombinant Saccharomyces cerevisiae Karyogamy protein KAR9 (KAR9), partial

  • 中文名称：
    酿酒酵母KAR9重组蛋白
  • 货号：
    CSB-YP341330SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母KAR9重组蛋白
  • 货号：
    CSB-EP341330SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母KAR9重组蛋白
  • 货号：
    CSB-EP341330SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母KAR9重组蛋白
  • 货号：
    CSB-BP341330SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母KAR9重组蛋白
  • 货号：
    CSB-MP341330SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KAR9
  • Uniprot No.：
    P32526
  • 别名：
    KAR9; YPL269W; Karyogamy protein KAR9; Cortical protein KAR9
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in karyogamy. Component of a cortical adaptor complex that orients cytoplasmic microtubules. It may be involved in anchoring cytoplasmic microtubules to the cell cortex.
  • 基因功能参考文献：
    1. KAR9 has microtubule end-binding motif LxxPTPh (where x and h represent any and hydrophobic amino acids, respectively). PMID: 28552577
    2. One crystal of Kar9p showed diffraction to 3 A resolution PMID: 23027760
    3. Kar9 is ubiquitylated and degraded by the proteasome after phosphorylation by Cdk1. PMID: 20598539
    4. Kar9p-mediated MT delivery along actin cables does not involve Bud6p at the cell cortex PMID: 15492045
    5. Overproduction of Bim1, but not Kar9, restores Kar9-dependent spindle positioning in the tub4-delta dsyl mutant, reduces cortical dwelling, and promotes Bim1-Kar9 interactions. PMID: 16899509
    6. Three independent mechanisms contribute to Kar9 asymmetry: Cdk1-dependent phosphorylation, sumoylation, and spindle-assembly checkpoint signaling. PMID: 18722122
    7. sumoylation regulates spindle positioning by restricting Kar9p to one spindle pole body. PMID: 18832349

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  • 亚细胞定位：
    Nucleus. Cytoplasm, cytoskeleton. Note=Localizes to the tip of shmoo projections and to the tip of budding cells in a cell-cycle dependent manner.
  • 数据库链接：

    KEGG: sce:YPL269W

    STRING: 4932.YPL269W

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