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  4. Recombinant Saccharomyces cerevisiae Inorganic phosphate transporter PHO84 (PHO84), partial

Recombinant Saccharomyces cerevisiae Inorganic phosphate transporter PHO84 (PHO84), partial

  • 中文名称：
    酿酒酵母PHO84重组蛋白
  • 货号：
    CSB-YP333061SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母PHO84重组蛋白
  • 货号：
    CSB-EP333061SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母PHO84重组蛋白
  • 货号：
    CSB-EP333061SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母PHO84重组蛋白
  • 货号：
    CSB-BP333061SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母PHO84重组蛋白
  • 货号：
    CSB-MP333061SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PHO84
  • Uniprot No.：
    P25297
  • 别名：
    PHO84; YML123C; YM7056.03C; Inorganic phosphate transporter PHO84
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    High-affinity transporter for external inorganic phosphate. Is not an essential protein, since a constitutive, low affinity pI transporter exists in yeast.
  • 基因功能参考文献：
    1. The phosphate-binding site residues were shown to be well conserved between all the plant PHT1 proteins and Saccharomyces cerevisiae PHO84. PMID: 27911737
    2. Molecular dynamics simulations of Pho84 in which a residue adjacent to Tyr(179), Asp(178), is protonated revealed a conformational change in Pho84 from an open, inward-facing state to an occluded state. PMID: 27875295
    3. The role of Pho84 in phosphate signalling is discussed. [review] PMID: 24845516
    4. PHO84 silencing uses antisense transcription via the promoter rather than the static accumulation of antisense RNAs at the repressed gene. PHO84 antisense RNA acts as a bimodal switch. PMID: 23770821
    5. trace levels of arsenate (70 ppb), the phosphate reduced the initial rate of uptake, but not the total amount removed. PHO84 mRNA levels were nearly 30 times higher in the engineered strains relative to the control strains PMID: 22628173
    6. Asp(358) (helix 7) and Lys(492) (helix 11) are critical for the transport function, and might be part of the putative substrate-binding pocket of Pho84. PMID: 22587366
    7. Pho84 functions as a sensor for glycerol-3-phosphate in Protein Kinase A signaling. PMID: 20133652
    8. We propose that Rpd3p has a negative role in the regulation of Pho84p endocytosis. PMID: 16087743
    9. A natural coding-region polymorphism in the inorganic phosphate transporter PHO84 underlies sensitivity to two polychlorinated phenols that uncouple oxidative phosphorylation. PMID: 17334364
    10. Chromatin cofactor requirements were determined by intrinsic nucleosome stabilities rather than correlated to promoter strength. PMID: 19307305
    11. Pho84-mediated activation of the PKA pathway is responsible for its own downregulation by phosphorylation, ubiquination, internalization, and vacuolar breakdown. PMID: 19348508

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Major facilitator superfamily, Sugar transporter (TC 2.A.1.1) family
  • 数据库链接：

    KEGG: sce:YML123C

    STRING: 4932.YML123C

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