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  4. Recombinant Saccharomyces cerevisiae Histone H2A.Z (HTZ1)

Recombinant Saccharomyces cerevisiae Histone H2A.Z (HTZ1)

  • 中文名称：
    酿酒酵母HTZ1重组蛋白
  • 货号：
    CSB-YP615527SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母HTZ1重组蛋白
  • 货号：
    CSB-EP615527SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母HTZ1重组蛋白
  • 货号：
    CSB-EP615527SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母HTZ1重组蛋白
  • 货号：
    CSB-BP615527SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母HTZ1重组蛋白
  • 货号：
    CSB-MP615527SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HTZ1
  • Uniprot No.：
    Q12692
  • 别名：
    HTZ1; H2AZ; HTA3; YOL012C; O2345; Histone H2A.Z
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-134
  • 氨基酸序列
    SGKAHGGKG KSGAKDSGSL RSQSSSARAG LQFPVGRIKR YLKRHATGRT RVGSKAAIYL TAVLEYLTAE VLELAGNAAK DLKVKRITPR HLQLAIRGDD ELDSLIRATI ASGGVLPHIN KALLLKVEKK GSKK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Variant histone H2A which can replace H2A in some nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. This variant is enriched at promoters, it may keep them in a repressed state until the appropriate activation signal is received. Near telomeres, it may counteract gene silencing caused by the spread of heterochromatin proteins. Required for the RNA polymerase II and SPT15/TBP recruitment to the target genes. Involved in chromosome stability.
  • 基因功能参考文献：
    1. Study shows that the H2A.Z histone variant impacts various aspects of meiotic development adding further insight into the relevance of chromatin dynamics for accurate gametogenesis. PMID: 29853474
    2. It has been found that the level of yeast histone variant H2A.Z in the G2/M-phase is actively kept low by the ubiquitin proteasome system and SUMO-targeted ubiquitin ligases. PMID: 28532323
    3. We found that ectopic CENP-A(Cse4) is enriched at promoters that contain histone H2A.Z(Htz1) nucleosomes, but that H2A.Z(Htz1) is not required for CENP-A(Cse4) mislocalization PMID: 26982580
    4. analysis suggests that Htz1 and nucleosome dynamics change in response to oleate stress. PMID: 26182431
    5. identify genetic links between Thr4 and the histone variant Htz1 and show that Thr4, as well as the Ino80 chromatin remodeler, is required for activation-associated eviction of Htz1 specifically from promoters of the Thr4-dependent genes PMID: 25071213
    6. nalysis of between-line variation shows the morphological effects of eliminating HTZ1 to be highly dependent on the line, which implies that HTZ1 interacts with mutations that have accumulated in the lines. PMID: 23990806
    7. The results indicates a function for Htz1 in promoting nucleotide excision repair in the Htz1-containing nucleosomes by regulating the occupancy of Gcn5 on chromatin, the UV-induced histone H3 acetylation and the binding of Rad14 to damaged DNA in the nucleosomes. PMID: 23925126
    8. Nucleosome-free region is critical for the targeting of multisubunit chromatin remodeler SWR1, it is not required for postrecruitment enzyme activity, which requires binding of the H2A.Z-H2B dimer. PMID: 24034247
    9. Nucleosomes containing Htz1p are primarily composed of H4 K12ac and H3 K4me3 but not H3 K36me3. PMID: 22393239
    10. The short forms of H2A.Z in both yeast and human cells are more loosely associated with chromatin than the full-length proteins, indicating a conserved function for the H2A.Z C-terminal tail in regulating the association of H2A.Z with nucleosomes. PMID: 22493515
    11. We verified an Swr1-independent role for H2A.Z following genotoxic stress in vivo. PMID: 21901086
    12. Here we have identified key functional regions located in the C-terminal docking domain in histone variant H2A.Z. PMID: 21791612
    13. Data suggest that residues F32 and I109 appear to have an Htz1-specific role. PMID: 21470346
    14. each Htz1 N-terminal lysine is deacetylated by Hda1 in response to benomyl and reacetylated when this agent is removed. Such active regulation suggests that acetylation plays a significant role in Htz1 function. PMID: 20952395
    15. NuA4-dependent acetylation of nucleosomal histones H4 and H2A directly stimulates incorporation of H2A.Z by the SWR1 complex. PMID: 20332092
    16. HTZ1 poises repressed/basal promoters for activation through histone loss. PMID: 16239141
    17. HTZ1 marks the 5' ends of both active and inactive genes in euchromatin. PMID: 16239142
    18. Swc2 binds directly to and is essential for transfer of H2AZ; the C-terminal alpha-helix of H2AZ is crucial for its recognition by SWR1. PMID: 16299513
    19. Htz1 may serve to mark quiescent promoters for proper activation PMID: 16344463
    20. cells lacking H2A.Z required the S-phase checkpoint pathway for survival and were delayed in the induction of cyclin genes. PMID: 16382141
    21. examination of role in transcription, DNA repair, chromosome stability, and limiting telomeric silencing PMID: 16543219
    22. results show a role for H2A.Z acetylation in restricting silent chromatin, and reveal that acetylation of H2A.Z and H4 can contribute to a common function essential to life PMID: 16543222
    23. H4 Lys 16 acetylation by SAS is required for efficient H2A.Z incorporation near telomeres. PMID: 16980580
    24. the DNA sequence of 322,000 individual Saccharomyces cerevisiae nucleosomes, containing the histone variant H2A.Z, to provide a comprehensive map of H2A.Z nucleosomes in functionally important regions PMID: 17392789
    25. H2A.Z and Set1 cooperate to prevent Sir-dependent repression of a large number of genes located across the genome, rather than the local effects reported previously for the individual mechanisms. PMID: 17925448
    26. Global dynamics of newly constructed oligonucleosomes of conventional and variant H2A.Z histone are reported. PMID: 17996059
    27. Results suggest that the initial presence of Htz1p appears to mark promoters for sustained gene expression and the recruitment of TATA-binding protein. PMID: 19273605
    28. nucleosome-free region establishment is necessary for H2A.Z deposition. These studies suggest an ordered pathway for the assembly of promoter chromatin architecture PMID: 19410542
    29. Data show that the variant histone, Htz1p, and its acetylation state seem to play an important role in maintaining ER retrieval pathways, suggesting a surprising link between chromatin remodeling and ER homeostasis. PMID: 19433630

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2A family
  • 数据库链接：

    KEGG: sce:YOL012C

    STRING: 4932.YOL012C

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