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  4. Recombinant Saccharomyces cerevisiae Glycerol-3-phosphate dehydrogenase [NAD (+)] 1

Recombinant Saccharomyces cerevisiae Glycerol-3-phosphate dehydrogenase [NAD (+)] 1

  • 中文名称：
    酿酒酵母GPD1重组蛋白
  • 货号：
    CSB-YP311439SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母GPD1重组蛋白
  • 货号：
    CSB-EP311439SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母GPD1重组蛋白
  • 货号：
    CSB-EP311439SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母GPD1重组蛋白
  • 货号：
    CSB-BP311439SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母GPD1重组蛋白
  • 货号：
    CSB-MP311439SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GPD1
  • Uniprot No.：
    Q00055
  • 别名：
    GPD1; DAR1; HOR1; OSG1; YDL022W; D2830Glycerol-3-phosphate dehydrogenase [NAD(+)] 1; EC 1.1.1.8
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-391
  • 氨基酸序列
    SAAADRLNL TSGHLNAGRK RSSSSVSLKA AEKPFKVTVI GSGNWGTTIA KVVAENCKGY PEVFAPIVQM WVFEEEINGE KLTEIINTRH QNVKYLPGIT LPDNLVANPD LIDSVKDVDI IVFNIPHQFL PRICSQLKGH VDSHVRAISC LKGFEVGAKG VQLLSSYITE ELGIQCGALS GANIATEVAQ EHWSETTVAY HIPKDFRGEG KDVDHKVLKA LFHRPYFHVS VIEDVAGISI CGALKNVVAL GCGFVEGLGW GNNASAAIQR VGLGEIIRFG QMFFPESREE TYYQESAGVA DLITTCAGGR NVKVARLMAT SGKDAWECEK ELLNGQSAQG LITCKEVHEW LETCGSVEDF PLFEAVYQIV YNNYPMKNLP DMIEELDLHE D
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the production and accumulation of glycerol during hyperosmotic stress conditions. Glycerol acts as a osmoregulator that prevents loss of water and turgor of the cells.
  • 基因功能参考文献：
    1. High expression of SIP18 at the beginning of rehydration was shown to be due to high transcription of the gene during the drying process. PMID: 28600909
    2. PUB1 mutant shows increased glycerol-3-phosphate dehydrogenase activity during wine making, which is related with an increase in the Gpd1 levels. PMID: 26846624
    3. Stress exposure results in increased peroxisomal levels of yeast Pnc1 and Gpd1, which are imported via a piggy-backing mechanism. PMID: 26516056
    4. GPD1 silencing gave 20 % less glycerol-3-phosphate dehydrogenase activity, 19 % lower glycerol production, and 9.7 % higher ethanol production compared with the original strain. PMID: 24150518
    5. The crystal structure of a glycerol-3-phosphate dehydrogenase, GPD1, from S. cerevisiae. PMID: 23143232
    6. Phosphorylation of Gpd1 and Gpd2 by distinct kinases enables rapid adaptation to specific stress conditions in Saccharomyces cerevisiae. PMID: 22988299

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  • 亚细胞定位：
    Cytoplasm. Peroxisome.
  • 蛋白家族：
    NAD-dependent glycerol-3-phosphate dehydrogenase family
  • 数据库链接：

    KEGG: sce:YDL022W

    STRING: 4932.YDL022W

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