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  4. Recombinant Saccharomyces cerevisiae GTP-binding protein YPT11 (YPT11)

Recombinant Saccharomyces cerevisiae GTP-binding protein YPT11 (YPT11)

  • 中文名称：
    酿酒酵母YPT11重组蛋白
  • 货号：
    CSB-YP342566SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母YPT11重组蛋白
  • 货号：
    CSB-EP342566SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母YPT11重组蛋白
  • 货号：
    CSB-EP342566SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母YPT11重组蛋白
  • 货号：
    CSB-BP342566SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母YPT11重组蛋白
  • 货号：
    CSB-MP342566SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    YPT11
  • Uniprot No.：
    P48559
  • 别名：
    YPT11; YNL304W; N0410; GTP-binding protein YPT11; Rab GTPase YPT11
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-417
  • 氨基酸序列
    MSQRKRYSLN VVTSPSIPSP TPSAPIRTNE SNWEAASPAS AASSFLPNVH HGGTVLNPGL GIMRSPSLNK SGAFGRSGSS GSSTVIEPSN IKLLLIGDAN VGKTAMILSY CRELLTRAEM SRSARLRHQQ QQQHKDLGLK KTVVNHRLSM KEKRKRYSSN DFEKEFKDIN HFADETSDFG NPNIGDDNNH EMADPNEIVI ETRSTIGIDI KTNLVNIDNR FFNVILWDTA GQERYQNAII PSLYKKTNAV ILTYDITNAK SFQSCMERWI VQALENFSSQ DLLKARFFLV GNKIDLYKER QVTHYDVVQM VQEMQLKHGI KISGNFEVSC KWVNVVERTM NMIILDLVEN GCFENNDPCV SITTSDDVQG HEQEFHDTVE EPFNFTRQRQ HQLEKNNTVD ITKPNDDIAN NQSICCV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the positive control of both endoplasmic reticulum (ER) and mitochondrion inheritance during cell divison. Required for the MYO2-dependent retention of newly inherited mitochondria at the bud tip in developing daughter cells.
  • 基因功能参考文献：
    1. The abundance of active Ypt11 forms is controlled by phosphorylation status and degradation. PMID: 23427260
    2. Myo2 drives anterograde mitochondrial movements in budding yeast and this activity is supported by Ypt11 and Mmr1. PMID: 21807878
    3. we show that, in human cells, after heterologous expression of GFP-Ypt11 (where GFP stands for green fluorescent protein), the protein is targeted to transferrin-positive recycling endosomes. PMID: 15850457
    4. required for the vectorial actin-dependent movement of the late Golgi from the mother cell toward the emerging bud PMID: 18595704
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Bud tip. Bud neck. Note=Enriched in the peripheral ER of small buds and daughter cells. Concentrates at the site of bud emergence, at the bud tip of the growing bud, and at the bud neck during M phase. Interaction with MYO2 is required for proper localization to the bud.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 数据库链接：

    KEGG: sce:YNL304W

    STRING: 4932.YNL304W

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