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  4. Recombinant Saccharomyces cerevisiae ERAD-associated E3 ubiquitin-protein ligase DOA10 (SSM4), partial

Recombinant Saccharomyces cerevisiae ERAD-associated E3 ubiquitin-protein ligase DOA10 (SSM4), partial

  • 中文名称：
    酿酒酵母SSM4重组蛋白
  • 货号：
    CSB-YP328097SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母SSM4重组蛋白
  • 货号：
    CSB-EP328097SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母SSM4重组蛋白
  • 货号：
    CSB-EP328097SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母SSM4重组蛋白
  • 货号：
    CSB-BP328097SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母SSM4重组蛋白
  • 货号：
    CSB-MP328097SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SSM4
  • Uniprot No.：
    P40318
  • 别名：
    SSM4; DOA10; YIL030C; YI3299.01C; YI9905.18CERAD-associated E3 ubiquitin-protein ligase DOA10; EC 2.3.2.27; RING-type E3 ubiquitin transferase DOA10
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC6 and UBC7 E2 ligases, and transfers it to substrates promoting their degradation. Mediates the degradation of a broad range of substrates, including endoplasmic reticulum membrane proteins (ERQC), soluble nuclear proteins and soluble cytoplasmic proteins (CytoQC). Component of the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains. ERAD-C substrates are ubiquitinated through DOA10 in conjunction with the E2 ubiquitin-conjugating enzymes UBC6 and UBC7-CUE1. Ubiquitinated substrates are then removed to the cytosol via the action of the UFD1-NPL4-CDC48/p97 (UNC) AAA ATPase complex and targeted to the proteasome. Also recognizes the N-terminally acetylated residue of proteins as degradation signal (degron). N-terminally acetylated target proteins include MATALPHA2, TBF1, SLK19, YMR090W, HIS3, HSP104, UBP6 and ARO8. Catalyzes ubiquitination of mislocalized tail-anchored proteins that are extracted from the mitochondrion membrane by MSP1: following extraction, mistargeted proteins are transferred to the endoplasmic retuculum, where they are ubiquitinated by DOA10 and degraded by the proteasome.
  • 基因功能参考文献：
    1. Sequential poly-ubiquitylation by specialized conjugating enzymes Ubc6 and Ubc7 expands the versatility of a quality control ubiquitin ligase Doa10. PMID: 27570077
    2. Here, the authors show that the ERAD ubiquitin ligase Doa10 controls the levels of some lipid droplet proteins. PMID: 27357570
    3. Data suggest that human MARCH6 and Saccharomyces cerevisiae Doa10 ubiquitin ligases are functionally similar. PMID: 27068744
    4. the TEB4-Doa10 domain regulates Doa10 association with the Ubc6 membrane anchor, thereby controlling the degradation rate of the E2. PMID: 21467040
    5. analysis of yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 PMID: 16373356
    6. Doa10 ubiquitinates both membrane and soluble proteins. PMID: 16437165
    7. demonstration that Doa10 reaches the inner nuclear membrane; localization of Doa10 to the inner nuclear membrane is necessary for nuclear substrate degradation PMID: 17051211

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    DOA10/MARCH6 family
  • 数据库链接：

    KEGG: sce:YIL030C

    STRING: 4932.YIL030C

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