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  4. Recombinant Saccharomyces cerevisiae DNA repair protein REV1 (REV1), partial

Recombinant Saccharomyces cerevisiae DNA repair protein REV1 (REV1), partial

  • 中文名称：
    酿酒酵母REV1重组蛋白
  • 货号：
    CSB-YP019576SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母REV1重组蛋白
  • 货号：
    CSB-EP019576SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母REV1重组蛋白
  • 货号：
    CSB-EP019576SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母REV1重组蛋白
  • 货号：
    CSB-BP019576SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母REV1重组蛋白
  • 货号：
    CSB-MP019576SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    REV1
  • Uniprot No.：
    P12689
  • 别名：
    REV1; YOR346W; O6339; DNA repair protein REV1; EC 2.7.7.-; Reversionless protein 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.
  • 基因功能参考文献：
    1. The structures of the translesion DNA synthesis polymerase Rev1 in complex with benzo[a]pyrene guanine adducts gives insights how Rev1 achieves error-free replication. PMID: 29042535
    2. Rev1-BRCT domain bound to PCNA can be displaced by increasing amounts of the PIP-box peptide. PMID: 23747975
    3. Rev1 stably interacts with Rad5 via the C-terminal region of Rev1 and the N-terminal region of Rad5. PMID: 23142547
    4. The study reports the X-ray crystal structure of the yeast Rev1 BRCT domain and show that substitutions in residues constituting its phosphate-binding pocket do not affect mutagenic translesion synthesis. PMID: 23240687
    5. the levels of Rev1 protein are dependent on proteasome-mediated degradation;proteasomal degradation acts as a regulatory system of mutagenic translesion DNA synthesis mediated by Rev1. PMID: 21227758
    6. results show that Rev1's catalytic activity is important in vivo when the cell has to cope with specific DNA lesions, such as N(2)-dG. PMID: 20980236
    7. We present here the structure of yeast Rev1 in ternary complex with DNA containing an abasic lesion and with dCTP as the incoming nucleotide. The structure shows a mechanism of synthesis across an abasic lesion that differs from that in other polymerases. PMID: 21167175
    8. Only the second ubiquitin-binding motif of Saccharomyces cerevisiae Rev1 binds to ubiquitin and is essential for Rev1-dependent cell survival and mutagenesis. PMID: 20159559
    9. Dbf4 and Rev1 BRCT domains interact with a common protein or structure, although the precise function of both domains and their binding partners remains elusive PMID: 19822727
    10. Strains deficient in REV1 protein show little bypass of the T-T (6-4) photoadduct or the abasic sites. PMID: 15520252
    11. REVIEW: REV1 is involved in somatic hypermutation and double strand break repair PMID: 15588843
    12. The polymerase domain of REV1 is responsible for the dCMP incorporation; the BRCT domain is important but dispensable for translation synthesis. PMID: 15896814
    13. crystal structure of Rev1 bound to template G and incoming 2'-deoxycytidine 5'-triphosphate (dCTP), which reveals that the polymerase itself dictates the identity of the incoming nucleotide, as well as the identity of the templating base PMID: 16195463
    14. DNA polymerase zeta and Rev1 proteins function in the mitochondria PMID: 16452144
    15. Data show that Saccharomyces cerevisiae Rev1 is subject to pronounced cell cycle control in which the levels of Rev1 protein are approximately 50-fold higher in G(2) and throughout mitosis than during G(1) and much of S phase. PMID: 16751278
    16. Results report that elevated levels of the yeast Rev1 C terminus confer a strong dominant-negative effect on viability and induced mutagenesis after DNA damage. PMID: 16923957
    17. Rev1 binding enhances the proficiency of DNA polymerase zeta for the extension of mismatched primer termini on undamaged DNAs and for the extension of primer termini opposite DNA lesions. PMID: 17030609
    18. pre-steady-state kinetic studies with Rev1p PMID: 17960914
    19. We further show that the minimal C-terminal fragment of Rev1 containing these highly conserved motifs is sufficient to interact with Rev7. PMID: 18603483
    20. Rev1 may be involved in facilitating mutagenic and recombinagenic responses to the failure of Pol delta. PMID: 18711219
    21. plays a role in mutagenesis and translesin DNA synthesis. (review) PMID: 18975621
    22. Results suggest that the proto-oncogene homologue Sch9 promotes the accumulation of superoxide-dependent DNA damage in nondividing cells by a superoxide and Rev1/Polzeta-dependent mechanism. PMID: 19687253

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  • 亚细胞定位：
    Nucleus. Mitochondrion.
  • 蛋白家族：
    DNA polymerase type-Y family
  • 数据库链接：

    KEGG: sce:YOR346W

    STRING: 4932.YOR346W

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