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  4. Recombinant Saccharomyces cerevisiae DNA repair protein RAD5 (RAD5), partial

Recombinant Saccharomyces cerevisiae DNA repair protein RAD5 (RAD5), partial

  • 中文名称：
    酿酒酵母RAD5重组蛋白
  • 货号：
    CSB-YP335773SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母RAD5重组蛋白
  • 货号：
    CSB-EP335773SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母RAD5重组蛋白
  • 货号：
    CSB-EP335773SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母RAD5重组蛋白
  • 货号：
    CSB-BP335773SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母RAD5重组蛋白
  • 货号：
    CSB-MP335773SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RAD5
  • Uniprot No.：
    P32849
  • 别名：
    RAD5; REV2; SNM2; YLR032W; DNA repair protein RAD5; EC 3.6.4.-; Radiation sensitivity protein 5; Revertibility protein 2
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable helicase, member of the UBC2/RAD6 epistasis group. Functions with the DNA repair protein RAD18 in error-free postreplication DNA repair. Involved in the maintenance of wild-type rates of instability of simple repetitive sequences such as poly(GT) repeats. Seems to be involved in maintaining a balance which acts in favor of error-prone non-homologous joining during DNA double-strand breaks repairs. Recruits the UBC13-MMS2 dimer to chromatin for DNA repair.
  • 基因功能参考文献：
    1. By analyzing the formation of Rad5 foci after MMS treatment, we showed that some specific DNA structures rather than mono-ubiquitination of proliferating cell nuclear antigen are required for the recruitment of Rad5 to the damaged site PMID: 29396601
    2. Rad5 Ub-ligase and ATPase/helicase activities are required for the response to DNA damage. PMID: 25310987
    3. Data indicate that the DNA helicase Rad5 ubiquitin ligase activity does not require ATP. PMID: 25690888
    4. Findings suggest a structural role of the helicase domain of Rad5 and the helicase activity is dispensable for error-free lesion bypass. PMID: 24674630
    5. A survey of various yeast deletion mutants converged on the Rad5 pathway of DNA damage tolerance by template switching as the likely target pathway of NSC109268 in mediating cellular sensitization to cisplatin. PMID: 24130896
    6. Rad5 stably interacts with Rev1 via the N-terminal region of Rad5 and the C-terminal region of Rev1. PMID: 23142547
    7. we genetically characterized a conserved yeast ICL repair pathway composed of the yeast homologs (Mph1, Chl1, Mhf1, Mhf2) of four FA proteins (FANCM, FANCJ, MHF1, MHF2 PMID: 22696213
    8. The authors demonstrate that in the absence of PCNA SUMOylation, Mms2-Ubc13 and Rad5 may, independently of each other, function in the stimulation of translesion DNA synthesis. PMID: 21362066
    9. Results indicate that multiple activities of Rad5 function coordinately with homologous recombination factors to enable replication template switch events that join sister chromatids at stalled forks and bypass DNA damage. PMID: 20541998
    10. RAD5 contributes to DNA double-strand break repair via the Mre11/Rad50/Xrs2 protein complex. PMID: 16224103
    11. In contrast to the role of Rad5 in postreplicational repair, however, where its function is coupled with that of Mms2-Ubc13, Rad5 function in translesion synthesis would be largely independent of this ubiquitin-conjugating enzyme complex PMID: 16908531
    12. Data show that yeast Rad5 has a DNA helicase activity that is specialized for replication fork regression. PMID: 17936713
    13. Rad5 is required for DNA polymerase zeta-dependent translesion synthesis in Saccharomyces cerevisiae PMID: 18757916
    14. Rad5 acts both to align monoUb-PCNA with Ub-charged Ubc13 and to stimulate Ub transfer onto Lys(63) of a Ub acceptor. PMID: 19706603

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 数据库链接：

    KEGG: sce:YLR032W

    STRING: 4932.YLR032W

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