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  4. Recombinant Saccharomyces cerevisiae DNA repair and recombination protein RAD26 (RAD26), partial

Recombinant Saccharomyces cerevisiae DNA repair and recombination protein RAD26 (RAD26), partial

  • 中文名称：
    酿酒酵母RAD26重组蛋白
  • 货号：
    CSB-YP328101SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母RAD26重组蛋白
  • 货号：
    CSB-EP328101SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母RAD26重组蛋白
  • 货号：
    CSB-EP328101SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母RAD26重组蛋白
  • 货号：
    CSB-BP328101SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母RAD26重组蛋白
  • 货号：
    CSB-MP328101SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RAD26
  • Uniprot No.：
    P40352
  • 别名：
    RAD26; GTA1085; YJR035W; J1606; DNA repair and recombination protein RAD26; EC 3.6.4.12; ATP-dependent helicase RAD26
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be involved in the preferential repair of active genes.
  • 基因功能参考文献：
    1. structure of the S. cerevisiae Pol II-Rad26 complex solved by cryo-electron microscopy; structure reveals that Rad26 binds to the DNA upstream of Pol II, where it markedly alters its path; structural and functional data suggest that the conserved Swi2/Snf2-family core ATPase domain promotes the forward movement of Pol II, and elucidate key roles for Rad26 in both TCR and transcription elongation PMID: 29168508
    2. Rad26 may facilitate transcription coupled nucleotide excision repair (TC-NER) indirectly, by antagonizing the action of TC-NER repressors that normally promote transcription elongation. PMID: 26429063
    3. Data indicate that RAD26 deletion does not reduce expression of nucleotide excision repair (NER) factors but affects RPB2 transcription during NER. PMID: 23991048
    4. Rad26p promotes the eviction of histone H2A-H2B dimer and hence chromatin disassembly. PMID: 22794311
    5. Rad26p does not recognize DNA lesions in the absence of active transcription. PMID: 20007604
    6. Spt5 CTR suppresses Rad26-independent TCR by serving as a platform for assembly of a multiple protein suppressor complex that is associated with Pol II. PMID: 20042611
    7. Rad26 plays a significant role in repairing certain regions of the repressed GAL1-10, PHO5 and ADH2 genes, especially in the core DNA of well-positioned nucleosomes PMID: 17611170

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 数据库链接：

    KEGG: sce:YJR035W

    STRING: 4932.YJR035W

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