Recombinant Saccharomyces cerevisiae Antiviral helicase SKI2 (SKI2), partial

中文名称：

酿酒酵母SKI2重组蛋白
货号：

CSB-YP327406SVG
规格：
来源：

Yeast
其他：

中文名称：

酿酒酵母SKI2重组蛋白
货号：

CSB-EP327406SVG
规格：
来源：

E.coli
其他：

中文名称：

酿酒酵母SKI2重组蛋白
货号：

CSB-EP327406SVG-B
规格：
来源：

E.coli
共轭：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名称：

酿酒酵母SKI2重组蛋白
货号：

CSB-BP327406SVG
规格：
来源：

Baculovirus
其他：

中文名称：

酿酒酵母SKI2重组蛋白
货号：

CSB-MP327406SVG
规格：
来源：

Mammalian cell
其他：

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纯度：

>85% (SDS-PAGE)
基因名：

SKI2
Uniprot No.：

P35207
别名：

SKI2; YLR398C; L8084.17; Antiviral helicase SKI2; EC 3.6.4.13; Superkiller protein 2
种属：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白长度：

Partial
蛋白标签：

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
产品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

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功能：

RNA helicase component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for cell growth only in the presence of M1 replicon.
基因功能参考文献：
1. Ski2, Ski3, and Ski8 assemble in a tetramer with 1:1:2 stoichiometry; 370 kDa core complex shows that Ski3 forms an array of 33 tetratricopeptide repeat motifs organized in N-terminal and C-terminal arms. The C-terminal arm of Ski3 and the two Ski8 subunits position the helicase core of Ski2 centrally within the complex, enhancing RNA binding. PMID: 23953113
2. the 2.4 A resolution crystal structure of the 113 kDa helicase region of Saccharomyces cerevisiae Ski2 was determined. PMID: 22114319
3. Inactivating the Ski complex by mutating conserved residues in the DEVH helicase motif of Ski2 did not abrogate its interaction with Ski7p, indicating that Ski2p function is not necessary for this interaction. PMID: 16043509
亚细胞定位：

Cytoplasm.
蛋白家族：

Helicase family, SKI2 subfamily
数据库链接：

KEGG: sce:YLR398C

STRING: 4932.YLR398C

Recombinant Saccharomyces cerevisiae Antiviral helicase SKI2 (SKI2), partial

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