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  4. Recombinant Saccharomyces cerevisiae APC/C activator protein CDH1 (CDH1), partial

Recombinant Saccharomyces cerevisiae APC/C activator protein CDH1 (CDH1), partial

  • 中文名称：
    Recombinant Saccharomyces cerevisiae APC/C activator protein CDH1(CDH1) ,partial
  • 货号：
    CSB-YP347390SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae APC/C activator protein CDH1(CDH1) ,partial
  • 货号：
    CSB-EP347390SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae APC/C activator protein CDH1(CDH1) ,partial
  • 货号：
    CSB-EP347390SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae APC/C activator protein CDH1(CDH1) ,partial
  • 货号：
    CSB-BP347390SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Saccharomyces cerevisiae APC/C activator protein CDH1(CDH1) ,partial
  • 货号：
    CSB-MP347390SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CDH1
  • Uniprot No.：
    P53197
  • 别名：
    CDH1; HCT1; YGL003CAPC/C activator protein CDH1; CDC20 homolog 1; Homolog of CDC twenty 1
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). During telophase and in the subsequent G1 phase of the cell cycle, recognizes and binds proteins containing a destruction box (D-box) and an additional degradation signal termed the KEN box including ASE1, CDC20, the B-type cyclins CLB2 and CLB3, the polo-like kinase CDC5 and HSL1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for exit from mitosis, cytokinesis and formation of prereplicative complexes in G1. Probably is the target of a BUB2-dependent spindle checkpoint pathway.
  • 基因功能参考文献：
    1. results suggest that binding to the D-box receptor may be a shared requirement for recognition and processing of all Cdh1 substrates. PMID: 27226622
    2. This study showed that the APC/C(Cdh1) ubiquitinates Ndd1 and mediates its degradation, and that APC/C(Cdh1) activity suppresses accumulation of Ndd1 targets. PMID: 25959309
    3. Cdh1 mediates Cdc20 degradation in metaphase, promoting formation of the APC/C-Cdh1. PMID: 25025567
    4. the structure of an Acm1-Cdh1 complex PMID: 23707760
    5. Acm1 is not required to inhibit APC(Cdh1) activity but rather prevents untimely Cdh1-substrate interactions. PMID: 22189709
    6. Tight Cdh1 binding combined with the inaccessibility of ubiquitinatable lysines contributes to pseudosubstrate inhibition of anaphase promoting complex(Cdh1). PMID: 21460798
    7. Data show that different spindle-regulatory pathways have distinct sensitivities to Cdh1, and ACM1 may buffer essential CDK phosphorylation of Cdh1. PMID: 20089834
    8. phosphorylation of CDK sites promotes the subsequent recognition of Cdh1 by at least one additional kinase PMID: 15467459
    9. Data demonstrate that Cdh1p interacts directly with the D box and the KEN box, two motifs within anaphase-promoting complex (APC) substrates known to be required for APC-mediated degradation. PMID: 15916960
    10. Data imply that the anaphase-promoting complex/cyclosome (APC/C) is activated by association of Cdh1 with Cdc27, which enables APC/C to recognize the D box of substrates via Cdh1's propeller domain. PMID: 15916961
    11. As cells complete mitosis and disassemble the spindle, the ubiqutin ligase APC(Cdh1) targets Fin1 for destruction. PMID: 17173039
    12. Cdh1-mediated degradation of the Polo kinase Cdc5 promotes the return of Cdc14 into the nucleolus. PMID: 18172166
    13. Data show that in budding yeast the activation of APC(Cdh1) is controlled in part by destruction of the Cdh1 inhibitor Acm1. PMID: 18498748
    14. unique property of the Acm1 degron sequences results from an unusually high affinity interaction with the substrate receptor site on the WD40 domain of Cdh1 that may serve both to promote APC inhibition and protect Acm1 from destruction. PMID: 18596038
    15. a role of G(1) specific APC/C(Cdh1) activity is to reset substrate levels to enables appropriate regulation of substrate accumulation in the subsequent phases of the cell cycle. PMID: 19713762

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Nuclear import and export are mediated by the importin PSE1 and the exportin MSN5.
  • 蛋白家族：
    WD repeat CDC20/Fizzy family
  • 数据库链接：

    KEGG: sce:YGL003C

    STRING: 4932.YGL003C

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