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  4. Recombinant Saccharomyces cerevisiae 78 kDa glucose-regulated protein homolog (KAR2), partial

Recombinant Saccharomyces cerevisiae 78 kDa glucose-regulated protein homolog (KAR2), partial

  • 中文名称：
    酿酒酵母KAR2重组蛋白
  • 货号：
    CSB-YP325426SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母KAR2重组蛋白
  • 货号：
    CSB-EP325426SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母KAR2重组蛋白
  • 货号：
    CSB-EP325426SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母KAR2重组蛋白
  • 货号：
    CSB-BP325426SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母KAR2重组蛋白
  • 货号：
    CSB-MP325426SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KAR2
  • Uniprot No.：
    P16474
  • 别名：
    KAR2; GRP78; SSD1; YJL034W; J1248Endoplasmic reticulum chaperone BiP; EC 3.6.4.10; 78 kDa glucose-regulated protein homolog; GRP-78; Immunoglobulin heavy chain-binding protein homolog; BiP
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis.
  • 基因功能参考文献：
    1. Reductive stress in the endoplasmic reticulum (ER) inhibits the Ost3-dependent N-glycosylation of Sil1, which regulates specific BiP functions appropriate to the needs of the ER under reductive stress. PMID: 29109265
    2. Here the authors demonstrate that BiP's nucleotide exchange factor - Sil1 - can reverse BiP cysteine oxidation. PMID: 28257000
    3. The authors found that the ATPase activity displayed by unmodified Kar2 is fully maintained when Cys63 is replaced with Ala or Val. PMID: 27543005
    4. the susceptibility of BiP to modification with glutathione may serve also to prevent irreversible oxidation of BiP by peroxide. PMID: 26865632
    5. Alteration of KAR2 (BiP) activity upon oxidation helps cells cope with disruption to oxidative folding within the endoplasmic reticulum during oxidative stress. PMID: 25053742
    6. Data indicate that BiP (GRP78/Kar2) stabilizes torsinA and torsinADeltaE in mammalian cells. PMID: 24627482
    7. The inability to induce KAR2 under stress had no effect on its known housekeeping functions. PMID: 22190740
    8. Kar2p mobility represents a powerful new tool capable of distinguishing between the different mechanisms leading to unfolded protein response activation in living cells. PMID: 22219379
    9. Structural analysis of the Sil1-Bip complex reveals the mechanism for Sil1 to function as a nucleotide-exchange factor PMID: 21675960
    10. A major BiP cochaperone in antibody folding, ERdj3, modulated the conformational space of BiP in a nucleotide-dependent manner, placing the lid subdomain in an open, protein-accepting state. PMID: 21217698
    11. Interactions between Kar2p and its nucleotide exchange factors Sil1p and Lhs1p are mechanistically distinct PMID: 20430899
    12. in the ER stress-sensory system BiP is not the principal determinant of Ire1 activity, but an adjustor for sensitivity to various stresses PMID: 15520230
    13. The introduction of Kar2p improves secretory proteins in Pichia pastoris secretion 4-7 times. PMID: 16889384
    14. glutamine 88 is part of or close to the Ire1p-binding site displayed on the ATP-bound conformation of Kar2p PMID: 17276461
    15. Study identified 87 Kar2p-secreting strains, including a number of known components in secretory protein modification and sorting. PMID: 19433630

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  • 亚细胞定位：
    Endoplasmic reticulum lumen.
  • 蛋白家族：
    Heat shock protein 70 family
  • 数据库链接：

    KEGG: sce:YJL034W

    STRING: 4932.YJL034W

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