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  4. Recombinant Saccharomyces cerevisiae 60S acidic ribosomal protein P0 (RPP0)

Recombinant Saccharomyces cerevisiae 60S acidic ribosomal protein P0 (RPP0)

  • 中文名称：
    酿酒酵母RPP0重组蛋白
  • 货号：
    CSB-YP020336SVG
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    酿酒酵母RPP0重组蛋白
  • 货号：
    CSB-EP020336SVG
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    酿酒酵母RPP0重组蛋白
  • 货号：
    CSB-EP020336SVG-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    酿酒酵母RPP0重组蛋白
  • 货号：
    CSB-BP020336SVG
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    酿酒酵母RPP0重组蛋白
  • 货号：
    CSB-MP020336SVG
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RPP0
  • Uniprot No.：
    P05317
  • 别名：
    RPP0; L10E; RPA0; RPL10E; RPLA0; YLR340W; L8300.8; 60S acidic ribosomal protein P0; A0; L10e; Large ribosomal subunit protein uL10
  • 种属：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-312
  • 氨基酸序列
    MGGIREKKAE YFAKLREYLE EYKSLFVVGV DNVSSQQMHE VRKELRGRAV VLMGKNTMVR RAIRGFLSDL PDFEKLLPFV KGNVGFVFTN EPLTEIKNVI VSNRVAAPAR AGAVAPEDIW VRAVNTGMEP GKTSFFQALG VPTKIARGTI EIVSDVKVVD AGNKVGQSEA SLLNLLNISP FTFGLTVVQV YDNGQVFPSS ILDITDEELV SHFVSAVSTI ASISLAIGYP TLPSVGHTLI NNYKDLLAVA IAASYHYPEI EDLVDRIENP EKYAAAAPAA TSAASGDAAP AEEAAAEEEE ESDDDMGFGL FD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. uL10 forms part of the P stalk that participates in recruiting G proteins to the ribosome.
  • 基因功能参考文献：
    1. Ricin A chain (RTA), which binds to the stalk to depurinate the sarcin/ricin loop, is used as a molecular probe in yeast mutants in which the binding site for one or the other dimer on ribosomal P0 was deleted. PMID: 24576056
    2. The reported results support that while most ribosomes contain a P1alpha/P2beta-P0-P1beta/P2alpha structure in normal conditions, the stalk assembly mechanism can generate alternative compositions, which have been previously detected in the cell. PMID: 22275522
    3. The model of the pentameric ribosomal stalk complex displays three distinct lobes, which may correspond to the individual P1-P2 heterodimers anchored to the C-terminal domain of the P0 protein 60S subunit. PMID: 22458705
    4. The authors have found that neither P1 nor P2 directly interact with P0 unless the endogenous heterodimer partner is being expressed in the cell. PMID: 20225338
    5. loss of function of ribosomal protein P0 results in a deficit in 60S subunits, which is apparently due to impaired rRNA processing of 27S precursors PMID: 19789271
    6. Ribosomal P0 protein participates in ribosomal stalk complex formation through interaction with P1A-P2B complex. PMID: 15866509
    7. structure of its 26S rRNA binding site PMID: 16164560
    8. Two short amino acid regions of P0 protein located at positions 199-230 and 231-258, to be responsible for independent binding of two dimers, P1A-P2B and P1B-P2A respectively. PMID: 16573688
    9. results indicate that Mrt4 and P0 bind to the same site in the 25S rRNA. PMID: 19346338
    10. Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0. PMID: 19797078

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Universal ribosomal protein uL10 family
  • 数据库链接：

    KEGG: sce:YLR340W

    STRING: 4932.YLR340W

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